ArticleProceedings of the National Academy of Sciences of the United States of America2006
Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
- Article
- CRISPR-Cas9 library screening approach for anti-cancer drug discovery: overview and perspectives.Theranostics · 2022Review
- Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior.Genes & development · 2021Article
- A comprehensive library of human transcription factors for cell fate engineering.Nature biotechnology · 2021Article
- Role of CITED2 in stem cells and cancer.Oncology letters · 2020Review
- PI3K in stemness regulation: from development to cancer.Biochemical Society transactions · 2020Review
- lncRNAs combine and crosstalk with NSPc1 in ATRA-induced differentiation of U87 glioma cells.Oncology letters · 2019Article
- Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming.Stem cell reports · 2019Article
- Phenotypic miRNA Screen Identifies miR-26b to Promote the Growth and Survival of Endothelial Cells.Molecular therapy. Nucleic acids · 2018Article
- Prioritising Causal Genes at Type 2 Diabetes Risk Loci.Current diabetes reports · 2017Review
- Microarray-based detection and expression analysis of new genes associated with drug resistance in ovarian cancer cell lines.Oncotarget · 2017Article
- Phenotypic screening with primary neurons to identify drug targets for regeneration and degeneration.Molecular and cellular neurosciences · 2017Review
- MicroRNA-140-5p regulates osteosarcoma chemoresistance by targeting HMGN5 and autophagy.Scientific reports · 2017Article
- CITED2 Cooperates with ISL1 and Promotes Cardiac Differentiation of Mouse Embryonic Stem Cells.Stem cell reports · 2016Article
- Hmgn5 functions downstream of Hoxa10 to regulate uterine decidualization in mice.Cell cycle (Georgetown, Tex.) · 2016Article
- Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells.Science (New York, N.Y.) · 2016Article
- The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.PloS one · 2016Article
- Article
- Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.Stem cells (Dayton, Ohio) · 2015Article
- The high-mobility group nucleosome-binding domain 5 is highly expressed in breast cancer and promotes the proliferation and invasion of breast cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem (ES) cells hold great promise for the future of medicine. To elucidate the molecular mechanisms that control ES cell self-renewal and differentiation, a comprehensive knowledge of the molecules involved in these processes is required. Here we describe an effective approach for genomewide identification of functionally active genes in ES cells. This approach combines genetic screens based on cDNA libraries with microarray detection methods to permit high-throughput functional analyses. We implement this strategy to identify genes whose overexpression can maintain phenotypic properties of undifferentiated mouse ES cells under differentiation-inducing conditions, specifically in the absence of leukemia inhibitory factor. The identified genes encode a variety of regulatory proteins whose function in ES cells was previously unknown. Moreover, our approach is capable of detecting genes whose overexpression promote differentiation or cell death. Overall, our studies establish a methodology for highly sensitive identification of genes that confer particular phenotypes on ES cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.