ArticleJournal of bacteriology2006
Posttranscriptional control of the Salmonella enterica flagellar hook protein FlgE.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- Active removal of inhibitory components drives the flagellar type 3 secretion-specificity switch.mBio · 2026Article
- Active removal of inhibitory components drives the flagellar Type III Secretion Specificity Switch.bioRxiv : the preprint server for biology · 2026Article
- Article
- A β-cap on the FliPQR protein-export channel acts as the cap for initial flagellar rod assembly.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Targeting early proximal-rod component substrate FlgB to FlhB for flagellar-type III secretion in Salmonella.PLoS genetics · 2022Article
- Article
- Control of membrane barrier during bacterial type-III protein secretion.Nature communications · 2021Article
- Review
- A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties.Infection and immunity · 2018Article
- Mechanism of type-III protein secretion: Regulation of FlhA conformation by a functionally critical charged-residue cluster.Molecular microbiology · 2017Article
- Biochemical Characterization of the Flagellar Rod Components of Rhodobacter sphaeroides: Properties and Interactions.Journal of bacteriology · 2016Article
- ATPase-independent type-III protein secretion in Salmonella enterica.PLoS genetics · 2014Article
- Comparative analysis of the secretion capability of early and late flagellar type III secretion substrates.Molecular microbiology · 2014Article
- Engineering of bacteria for the visualization of targeted delivery of a cytolytic anticancer agent.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Molecular characterization of the flagellar hook in Bacillus subtilis.Journal of bacteriology · 2012Article
- A single-domain FlgJ contributes to flagellar hook and filament formation in the Lyme disease spirochete Borrelia burgdorferi.Journal of bacteriology · 2012Article
- Article
- CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.Molecular microbiology · 2011Article
- The role of the FliK molecular ruler in hook-length control in Salmonella enterica.Molecular microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous work suggested that the FlgE (flagellar hook subunit) protein in Salmonella enterica serovar Typhimurium was posttranscriptionally regulated in response to the stage of flagellar assembly. Specifically, the FlgE protein could be detected in flagellar mutants defective at the stages of assembly before or after rod assembly but not in rod assembly mutants, yet flgE mRNA levels were unaffected. To elucidate posttranscriptional mechanisms involved in the coupling of flgE gene expression to hook assembly, the RNA sequences at the 5' and 3' ends of the flgE-containing mRNA processed from the large flgBCDEFGHIJKL operon were determined by rapid amplification of cDNA ends, and secretion of the FlgE protein in different flagellar assembly mutant strains was analyzed. The sequences 5' and 3' of the flgE gene where RNA processing occurred was within 15 bases upstream of the flgD stop codon and at bases 145 to 147 downstream of the flgF start codon, respectively. The ribosome binding site of the flgD gene was found to be inhibitory to flgE translation in strains deleted for the upstream flgD gene, unless the region 15 bases upstream of the flgD stop codon was present. Secretion of FlgE into the periplasm was monitored using beta-lactamase (Bla) fusions as a periplasm-specific reporter, which conferred resistance to ampicillin when FlgE-Bla was secreted into the periplasm. Using this assay, we found that the effect of rod assembly mutants on FlgE levels was due to FlgE turnover in the periplasm and that the FliE rod component protein was required for efficient FlgE-Bla secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.