ArticleJournal of bacteriology2006
Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipooligosaccharide and capsular carbohydrate.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- Enzymatic Synthesis of an Undecorated Capsular Polysaccharide fromBiochemistry · 2025Article
- Identification and Functional Characterization of the Polymerizing Glycosyltransferase Required for the Transfer of d-Ribose to the d-GalBiochemistry · 2025Article
- Identification of the Polymerizing Glycosyltransferase Required for the Addition of d-Glucuronic Acid to the Capsular Polysaccharide ofBiochemistry · 2025Article
- Biosynthesis of UDP-α-Biochemistry · 2024Article
- Metabolic Pathways for the Biosynthesis of Heptoses Used in the Construction of Capsular Polysaccharides in the Human PathogenBiochemistry · 2023Article
- CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers.Applied microbiology and biotechnology · 2023Article
- Lipopolysaccharide structure modulates cationic biocide susceptibility and crystalline biofilm formation inFrontiers in microbiology · 2023Article
- Opportunities and Challenges of Bacterial Glycosylation for the Development of Novel Antibacterial Strategies.Frontiers in microbiology · 2021Review
- An Updated Classification System and Review of the Lipooligosaccharide Biosynthesis Gene Locus inFrontiers in microbiology · 2020Review
- Synthesis, kinetics and inhibition of Escherichia coli Heptosyltransferase I by monosaccharide analogues of Lipid A.Bioorganic & medicinal chemistry letters · 2018Article
- Phase-Variable Changes in the Position ofJournal of bacteriology · 2017Article
- Characterization of the dTDP-Fuc3N and dTDP-Qui3N biosynthetic pathways in Campylobacter jejuni 81116.Glycobiology · 2017Article
- Characterization of the emerging zoonotic pathogen Arcobacter thereius by whole genome sequencing and comparative genomics.PloS one · 2017Article
- Pancreatic amylase is an environmental signal for regulation of biofilm formation and host interaction in Campylobacter jejuni.Infection and immunity · 2015Article
- Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages.PloS one · 2015Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- Construction and characterization of an Escherichia coli mutant producing Kdo₂-lipid A.Marine drugs · 2014Article
- Biological roles of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni.PloS one · 2014Article
- Effects of lipooligosaccharide inner core truncation on bile resistance and chick colonization by Campylobacter jejuni.PloS one · 2013Article
- Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process.Current microbiology · 2012Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertional mutagenesis into the waaC gene, encoding heptosyltransferase I that catalyzes the transfer of the first L-glycero-D-manno-heptose residue to 3-deoxy-D-manno-octulosonic residue (Kdo)-lipid A. Tricine gel electrophoresis, followed by silver staining, showed that site-specific mutation in the waaC gene resulted in the expression of a severely truncated LOS compared to wild-type strain 81-176. Gas-liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy showed that the waaC LOS species lacked all sugars distal to Kdo-lipid A. Parallel structural studies of the capsular polysaccharides of the wild-type strain 81-176 and waaC mutant revealed loss of the 3-O-methyl group in the waaC mutant. Complementation of the C. jejuni mutant by insertion of the wild-type C. jejuni waaC gene into a chromosomal locus resulted in LOS and capsular structures identical to those expressed in the parent strain. We also report here the presence of O-methyl phosphoramidate in wild-type strain 81-176 capsular polysaccharide.
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