ArticleJournal of bacteriology2006
Regulatory roles for IscA and SufA in iron homeostasis and redox stress responses in the cyanobacterium Synechococcus sp. strain PCC 7002.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 90 citations in OpenAlex.
- Interactions between the nitrogen-fixing cyanobacteriumISME communications · 2024Article
- Dps Functions as a Key Player in Bacterial Iron Homeostasis.ACS omega · 2023Review
- TheMicroorganisms · 2023Article
- Complete genome assembly of Hawai'i environmental nontuberculous mycobacteria reveals unexpected co-isolation with methylobacteria.PloS one · 2023Article
- Spectroscopic and functional characterization of the [2Fe-2S] scaffold protein Nfu from Synechocystis PCC6803.Biochimie · 2022Article
- The Multifaceted Bacterial Cysteine Desulfurases: From Metabolism to Pathogenesis.Antioxidants (Basel, Switzerland) · 2021Review
- Regulation and Functional Complexity of the Chlorophyll-Binding Protein IsiA.Frontiers in microbiology · 2021Review
- From the Ocean to the Lab-Assessing Iron Limitation in Cyanobacteria: An Interface Paper.Microorganisms · 2020Review
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria.Frontiers in microbiology · 2020Review
- Cyanobacterial Siderophores-Physiology, Structure, Biosynthesis, and Applications.Marine drugs · 2019Review
- Diverse light responses of cyanobacteria mediated by phytochrome superfamily photoreceptors.Nature reviews. Microbiology · 2019Review
- Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.Molecular microbiology · 2018Article
- Presence of a [3Fe-4S] cluster in a PsaC variant as a functional component of the photosystem I electron transfer chain in Synechococcus sp. PCC 7002.Photosynthesis research · 2018Article
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- Mapping cellular Fe-S cluster uptake and exchange reactions - divergent pathways for iron-sulfur cluster delivery to human ferredoxins.Metallomics : integrated biometal science · 2016Article
- Cytosolic iron-sulfur cluster transfer-a proposed kinetic pathway for reconstitution of glutaredoxin 3.FEBS letters · 2016Article
- Nfu facilitates the maturation of iron-sulfur proteins and participates in virulence in Staphylococcus aureus.Molecular microbiology · 2015Article
- An Ipomoea batatas iron-sulfur cluster scaffold protein gene, IbNFU1, is involved in salt tolerance.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, and nfu. Demonstrating the nonessential nature of their products, the sufA, iscA, and sufA iscA mutants grew photoautotrophically with doubling times that were similar to the wild type under standard growth conditions. In contrast, attempts to inactivate the nfu gene only resulted in stable merodiploids. These results imply that Nfu, but not SufA or IscA, is the essential Fe/S scaffold protein in cyanobacteria. When cells were grown under iron-limiting conditions, the iscA and sufA mutant strains exhibited less chlorosis than the wild type. Under iron-sufficient growth conditions, isiA transcript levels, a marker for iron limitation in cyanobacteria, as well as transcript levels of genes in both the suf and isc regulons were significantly higher in the iscA mutant than in the wild type. Under photosynthesis-induced redox stress conditions, the transcript levels of the suf genes are notably higher in the sufA and the sufA iscA mutants than in the wild type. The growth phenotypes and mRNA abundance patterns of the mutant strains contradict the proposed scaffold function for the SufA and IscA proteins in generalized Fe/S cluster assembly and instead suggest that they play regulatory roles in iron homeostasis and the sensing of redox stress in cyanobacteria.
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