ArticleBMC microbiology2006
Permeabilization of the mycobacterial envelope for protein cytolocalization studies by immunofluorescence microscopy.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals.Veterinary sciences · 2024Review
- The Mycobacterium tuberculosis PE15/PPE20 complex transports calcium across the outer membrane.PLoS biology · 2022Article
- Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria.Scientific reports · 2021Article
- Article
- NapM enhances the survival ofCommunications biology · 2019Article
- Structural and Functional Characterization of the Type Three Secretion System (T3SS) Needle ofFrontiers in microbiology · 2019Article
- FISHing Mycobacterium tuberculosis Complex by Use of aJournal of clinical microbiology · 2018Article
- Rv0004 is a new essential member of the mycobacterial DNA replication machinery.PLoS genetics · 2017Article
- Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin.Journal of clinical laboratory analysis · 2011Article
- A novel nucleoid-associated protein of Mycobacterium tuberculosis is a sequence homolog of GroEL.Nucleic acids research · 2009Article
- A PCR-based method for monitoring Legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe establishment of the cellular localization of proteins in M. tuberculosis will provide of valuable information for the identification of new drug/vaccine/diagnostic targets. Cytolocalization by inmunofluorescence microscopy has been limited in mycobacteria because to difficulties in effectively permeabilize it.
resultsA treatment combining lysozyme with triton X-100 was found to be an effective permeabilization method of the mycobacterial envelope.
conclusionA rapid and simple permeabilization protocol has been successfully assessed in pure cultures of both Mycobacterium smegmatis and Mycobacterium tuberculosis H37Rv. This method can be successful used in the cytolocalization of proteins by immunolabeling.
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