ArticleGenes & development2006
A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 202 papers.
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Who cites it
202 citing papers in PubMed, 404 citations in OpenAlex.
- More than SAM: mechanisms of action of the Polycomb group protein polyhomeotic.Nucleus (Austin, Tex.) · 2026Review
- Polycomb Group Proteins inBiology · 2026Review
- Article
- A maternal Polycomb imprint is maintained in early Drosophila development through lower-order methylation states.Cell reports · 2026Article
- Foxh1 is a locus-specific PRC2 recruiter governing germ layer silencing.bioRxiv : the preprint server for biology · 2025Article
- Lower-order methylation states underlie the maintenance and re-establishment of Polycomb modifications inbioRxiv : the preprint server for biology · 2025Article
- Differential regulation of eye specification in Drosophila by Polycomb Group epigenetic repressors.Development (Cambridge, England) · 2025Article
- Article
- A nuclear architecture screen inGenes & development · 2024Article
- Disruption of maternal vascular remodeling by a fetal endoretrovirus-derived gene in preeclampsia.Genome biology · 2024Article
- Polycomb protein binding and looping in the ON transcriptional state.Science advances · 2024Article
- Lysine Methylation-Dependent Proteolysis by the Malignant Brain Tumor (MBT) Domain Proteins.International journal of molecular sciences · 2024Review
- A nuclear architecture screen inbioRxiv : the preprint server for biology · 2023Article
- Polycomb protein binding and looping mediated by Polycomb Response Elements in the ON transcriptional state.bioRxiv : the preprint server for biology · 2023Article
- Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila.Genetics · 2023Article
- Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Crol contributes to PRE-mediated repression and Polycomb group proteins recruitment in Drosophila.Nucleic acids research · 2023Article
- Genetic and Epigenetic Regulation ofJournal of developmental biology · 2023Review
- Nuclear architecture and the structural basis of mitotic memory.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
142 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polycomb response elements (PREs) are specific cis-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in Drosophila, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho, we purified Pho protein complexes from Drosophila embryos and found that Pho exists in two distinct protein assemblies: a Pho-dINO80 complex containing the Drosophila INO80 nucleosome-remodeling complex, and a Pho-repressive complex (PhoRC) containing the uncharacterized gene product dSfmbt. Analysis of PhoRC reveals that dSfmbt is a novel PcG protein that is essential for HOX gene repression in Drosophila. PhoRC is bound at HOX gene PREs in vivo, and this targeting strictly depends on Pho-binding sites. Characterization of dSfmbt protein shows that its MBT repeats have unique discriminatory binding activity for methylated lysine residues in histones H3 and H4; the MBT repeats bind mono- and di-methylated H3-K9 and H4-K20 but fail to interact with these residues if they are unmodified or tri-methylated. Our results establish PhoRC as a novel Drosophila PcG protein complex that combines DNA-targeting activity (Pho) with a unique modified histone-binding activity (dSfmbt). We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.