ArticleThe Journal of cell biology1991
Direct observation of actin filament severing by gelsolin and binding by gCap39 and CapZ.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- A Cell Density-Dependent Reporter in the Drosophila S2 Cells.Scientific reports · 2019Article
- Real-Time Single-Molecule Kinetic Analyses of AIP1-Enhanced Actin Filament Severing in the Presence of Cofilin.Journal of molecular biology · 2019Article
- Pivotal and distinct role for Plasmodium actin capping protein alpha during blood infection of the malaria parasite.Molecular microbiology · 2015Article
- Molecular profiling of single Sca-1+/CD34+,- cells--the putative murine lung stem cells.PloS one · 2013Article
- Mechanism for CARMIL protein inhibition of heterodimeric actin-capping protein.The Journal of biological chemistry · 2012Article
- Conformational dynamics of actin: effectors and implications for biological function.Cytoskeleton (Hoboken, N.J.) · 2010Review
- Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover.The Plant cell · 2010Article
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
- Overview of image analysis, image importing, and image processing using freeware.Current protocols in molecular biology · 2003Article
- Article
- Actin dynamics in platelets.International review of cytology · 2002Review
- Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations.Cell motility and the cytoskeleton · 2000Article
- Calcium influx alters actin bundle dynamics and retrograde flow in Helisoma growth cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- 2E4 (kaptin): a novel actin-associated protein from human blood platelets found in lamellipodia and the tips of the stereocilia of the inner ear.European journal of cell biology · 1999Article
- Determination of the gelsolin binding site on F-actin: implications for severing and capping.Biophysical journal · 1998Article
- An axoplasmic myosin with a calmodulin-like light chain.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Actin-based motility of isolated axoplasmic organelles.Cell motility and the cytoskeleton · 1996Article
- The ADF/cofilin proteins: stimulus-responsive modulators of actin dynamics.Molecular biology of the cell · 1995Review
- Cytoskeletal domains in the activated platelet.Cell motility and the cytoskeleton · 1995Article
Corrections and comments
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1 author at 1 institution in 1 country.
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Abstract
Dynamic behavior of actin filaments in cells is the basis of many different cellular activities. Remodeling of the actin filament network involves polymerization and depolymerization of the filaments. Proteins that regulate these behaviors include proteins that sever and/or cap actin filaments. This report presents direct observation of severing of fluorescently-labeled actin filaments. Coverslips coated with gelsolin, a multi-domain, calcium-dependent capping and severing protein, bound rhodamine-phalloidin-saturated filaments along their length in the presence of EGTA. Upon addition of calcium, attached filaments bent as they broke. Actophorin, a low molecular weight, monomer sequestering, calcium-independent severing protein did not sever phalloidin-saturated filaments. Both gCap 39, a gelsolin-like, calcium-dependent capping protein that does not sever filaments, and CapZ, a heterodimeric, non-calcium-dependent capping protein, bound the filaments by one end to the coverslip. Visualization of individual filaments also revealed severing activity present in mixtures of actin-binding proteins isolated by filamentous actin affinity chromatography from early Drosophila embryos. This activity was different from either gelsolin or actophorin because it was not inhibited by phalloidin, but was calcium independent. The results of these studies provide new information about the molecular mechanisms of severing and capping by well-characterized proteins as well as definition of a novel type of severing activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.