ArticleNucleic acids research2006
A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Photothermal Recycling Biosensing for Continuous, Sensitive Molecular Quantification.bioRxiv : the preprint server for biology · 2026Article
- Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins.Scientific reports · 2021Article
- Article
- A Broadly Applicable Assay for Rapidly and Accurately Quantifying DNA Surface Coverage on Diverse Particles.Bioconjugate chemistry · 2017Article
- Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Chemical basis of interactions between engineered nanoparticles and biological systems.Chemical reviews · 2014Review
- Quantification of functionalised gold nanoparticle-targeted knockdown of gene expression in HeLa cells.PloS one · 2014Article
- Colorimetric Assay for Exon 7 SMN1/SMN2 Single Nucleotide Polymorphism Using Gold Nanoprobes.BioImpacts : BI · 2013Article
- Ligand synthesis and passivation for silver and large gold nanoparticles for single-particle-based sensing and spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Towards in vitro molecular diagnostics using nanostructures.Cellular and molecular life sciences : CMLS · 2012Review
- A highly sensitive detection for foot-and-mouth disease virus by gold nanopariticle improved immuno-PCR.Virology journal · 2011Article
- Detection of HIV-1 p24 Gag in plasma by a nanoparticle-based bio-barcode-amplification method.Nanomedicine (London, England) · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Here we report a real-time PCR-based method for determining the surface coverage of dithiol-capped oligonucleotides bound onto gold nanoparticles alone and in tandem with antibody. The detection of gold nanoparticle-bound DNA is accomplished by targeting the oligonucleotide with primer and probe binding sites, amplification of the oligonucleotide by PCR, and real-time measurement of the fluorescence emitted during the reaction. This method offers a wide dynamic range and is not dependant on the dissociation of the oligonucleotide strands from the gold nanoparticle surface; the fluorophore is not highly quenched by the gold nanoparticles in solution during fluorescence measurements. We show that this method and a fluorescence-based method give equivalent results for determining the surface coverage of oligonucleotides bound onto 13 or 30 nm gold nanoparticles alone and in tandem with antibody. Quantifying the surface coverage of immobilized oligonucleotides on metallic nanoparticle surfaces is important for optimizing the sensitivity of gold nanoparticle-based detection methods and for better understanding the interactions between thiol-functionalized oligonucleotides and gold nanoparticles.
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Registered trials
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