ArticleProceedings of the National Academy of Sciences of the United States of America2006
Evidence on the chromosomal location of centromeric DNA in Plasmodium falciparum from etoposide-mediated topoisomerase-II cleavage.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Independent evolution of holocentric chromosomes in an early branching apicomplexan parasite.bioRxiv : the preprint server for biology · 2025Article
- A tale of topoisomerases and the knotty genetic material in the backdrop of Plasmodium biology.Bioscience reports · 2022Review
- Site-Specific Cleavage by Topoisomerase 2: A Mark of the Core Centromere.International journal of molecular sciences · 2018Article
- Identification of the centromeres ofEMBO reports · 2017Article
- Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity.PLoS pathogens · 2017Review
- Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.Scientific reports · 2016Article
- Topoisomerase II from Human Malaria Parasites: EXPRESSION, PURIFICATION, AND SELECTIVE INHIBITION.The Journal of biological chemistry · 2015Article
- Cell division in apicomplexan parasites.Nature reviews. Microbiology · 2014Review
- Review
- Radiation-induced cellular and molecular alterations in asexual intraerythrocytic Plasmodium falciparum.The Journal of infectious diseases · 2013Article
- How do microbial pathogens make CENs?PLoS pathogens · 2012Article
- Centromere-associated repeat arrays on Trypanosoma brucei chromosomes are much more extensive than predicted.BMC genomics · 2012Article
- A view on the role of epigenetics in the biology of malaria parasites.PLoS pathogens · 2012Review
- Toxoplasma gondii sequesters centromeres to a specific nuclear region throughout the cell cycle.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei.Nucleic acids research · 2011Article
- High recombination rates and hotspots in a Plasmodium falciparum genetic cross.Genome biology · 2011Article
- DNA topoisomerases in apicomplexan parasites: promising targets for drug discovery.Proceedings. Biological sciences · 2010Review
- A nuclear targeting system in Plasmodium falciparum.Malaria journal · 2010Article
- Functional identification of the Plasmodium centromere and generation of a Plasmodium artificial chromosome.Cell host & microbe · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Centromeres are the chromosomal loci that facilitate segregation, and, in most eukaryotes, they encompass extensive regions of genomic DNA. Topoisomerase-II has been identified as a crucial regulator of segregation in a wide range of organisms and exhibits premitotic accumulation at centromeres. Consistent with this property, treatment of cells with the topoisomerase-II inhibitor etoposide promotes chromosomal cleavage at sites within centromeric DNA. In the case of the human malaria parasite Plasmodium falciparum, despite a completed genome sequence, there are no experimental data on the nature of centromeres. To address this issue, we have used etoposide-mediated topoisomerase-II cleavage as a biochemical marker to map centromeric DNA on all 14 parasite chromosomes. We find that topoisomerase-II activity is concentrated at single chromosomal loci and that cleavage sites extend over approximately 10 kb. A shared feature of these topoisomerase-II cleavage sites is the presence of an extremely AT-rich ( approximately 97%) domain with a strictly defined size limit of 2.3-2.5 kb. Repetitive arrays identified within the domains do not display interchromosomal conservation in terms of length, copy number, or sequence. These unusual properties suggest that P. falciparum chromosomes contain a class of "regional" centromere distinct from those described in other eukaryotes, including the human host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.