ArticleThe Plant cell2006
Multiple vacuolar sorting determinants exist in soybean 11S globulin.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- Structural insights into how vacuolar sorting receptor recognizes the C-terminal sorting determinant of a vicilin-like seed storage protein.The FEBS journal · 2026Article
- Genetic mapping and functional genomics of soybean seed protein.Molecular breeding : new strategies in plant improvement · 2023Article
- Wheat α-gliadin and high-molecular-weight glutenin subunit accumulate in different storage compartments of transgenic soybean seed.Transgenic research · 2022Article
- Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A vacuolar sorting receptor-independent sorting mechanism for storage vacuoles in soybean seeds.Scientific reports · 2018Article
- Multiple internal sorting determinants can contribute to the trafficking of cruciferin to protein storage vacuoles.Plant molecular biology · 2015Article
- Biogenesis of protein bodies during vicilin accumulation in Medicago truncatula immature seeds.BMC research notes · 2012Article
- Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean.Journal of experimental botany · 2012Article
- A fluorescent reporter protein containing AtRMR1 domains is targeted to the storage and central vacuoles in Arabidopsis thaliana and tobacco leaf cells.Plant cell reports · 2011Article
- Sorting and anterograde trafficking at the Golgi apparatus.Plant physiology · 2008Article
- Fluorescent reporter proteins for the tonoplast and the vacuolar lumen identify a single vacuolar compartment in Arabidopsis cells.Plant physiology · 2007Article
- Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sorting determinants of glycinin, a soybean (Glycine max) 11S globulin, which mediates protein targeting to the protein storage vacuole (PSV), were investigated in maturing soybean cotyledons by transient expression assays. A C-terminal stretch of 10 amino acids of A1aB1b, a glycinin group I subunit, was sufficient to direct green fluorescent protein (GFP) to the PSV. This peptide may correspond to a C-terminal vacuolar sorting determinant (ctVSD). Because functional inhibition of this putative ctVSD of A1aB1b did not block PSV sorting of A1aB1b, we used the three-dimensional structure of A1aB1b to identify candidates for a sequence-specific determinant (ssVSD). We found that the sequence downstream of disordered region 4 could direct GFP to the PSV and that Ile-297 is critical for sorting. However, functional inhibition of the ctVSD, combined with the Ile297Gly mutation, did not abolish the vacuolar sorting of A1aB1b, suggesting that A1aB1b has a third sorting determinant in addition to ctVSD and ssVSD. A glycinin group II subunit, A3B4, lacked a ctVSD but contained a VSD reminiscent of an ssVSD and an additional sorting determinant. We also demonstrate, by expression of dominant negative mutants of small GTPases and drug treatment experiments, that the trafficking of A1aB1b is COPII vesicle-dependent and wortmannin- and brefeldin A-sensitive.
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