ArticleBiophysical journal2006
Measuring optical and mechanical properties of a living cell with defocusing microscopy.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
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- Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.Science advances · 2022Article
- Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.Nature communications · 2021Article
- Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro.Biophysical journal · 2021Article
- Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling.Scientific reports · 2021Article
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- The Effects of Shear Force Transmission Across Vesicle Membranes.The journal of physical chemistry letters · 2017Article
- Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2017Article
- Epidermal-specific deletion of CD44 reveals a function in keratinocytes in response to mechanical stress.Cell death & disease · 2016Article
- Anti-erythrocyte antibodies may contribute to anaemia in Plasmodium vivax malaria by decreasing red blood cell deformability and increasing erythrophagocytosis.Malaria journal · 2016Article
- Quantifying adhesion mechanisms and dynamics of human hematopoietic stem and progenitor cells.Scientific reports · 2015Article
- Mesoscale computational studies of membrane bilayer remodeling by curvature-inducing proteins.Physics reports · 2014Article
- Membrane cholesterol removal changes mechanical properties of cells and induces secretion of a specific pool of lysosomes.PloS one · 2013Article
- Membrane dynamics correlate with formation of signaling clusters during cell spreading.Biophysical journal · 2012Article
- Effects of cholesterol on nano-mechanical properties of the living cell plasma membrane.Soft matter · 2012Article
- Cell cytoskeleton and tether extraction.Biophysical journal · 2011Article
- Cell spreading as a hydrodynamic process.Soft matter · 2010Article
- Drag force as a tool to test the active mechanical response of PC12 neurites.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Defocusing microscopy (DM) is a recently developed technique that allows quantitative analysis of membrane surface dynamics of living cells using a simple bright-field optical microscope. According to DM, the contrast of defocused images is proportional to cell surface curvature. Although, until now, this technique was used mainly to determine size and amount of membrane shape fluctuations, such as ruffles and small random membrane fluctuations, in macrophages, its applications on cell biology extend beyond that. We show how DM can be used to measure optical and mechanical properties of a living macrophage, such as cell refractive index n, membrane bending modulus K(c), and effective cell viscosity eta for membrane-actin meshwork relaxation. Experimental data collected from defocused images of bone marrow-derived macrophages were used to evaluate these parameters. The obtained values, averaged over several different macrophages, are n = (1.384 +/- 0.015), K(c) approximately 3.2 x 10(-19) J, and eta approximately 459 Pa.s. We also estimate the amplitude of the small fluctuations to be of the order of 3 nm, which is around the step size of a polymerizing actin filament.
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