ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- An assessment of transgenomics as a tool for gene discovery in Populus euphratica Oliv.Plant molecular biology · 2018Article
- Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector.Journal of visualized experiments : JoVE · 2018Article
- Transformation of rice with large maize genomic DNA fragments containing high content repetitive sequences.Plant cell reports · 2015Article
- Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives.Genetics · 2013Article
- Construction of a plant-transformation-competent BIBAC library and genome sequence analysis of polyploid Upland cotton (Gossypium hirsutum L.).BMC genomics · 2013Article
- Toward a molecular cytogenetic map for cultivated sunflower (Helianthus annuus L.) by landed BAC/BIBAC clones.G3 (Bethesda, Md.) · 2013Article
- Molecular mapping of the Pl(16) downy mildew resistance gene from HA-R4 to facilitate marker-assisted selection in sunflower.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange.Plant methods · 2011Article
- sunTILL: a TILLING resource for gene function analysis in sunflower.Plant methods · 2011Article
- Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae).BMC research notes · 2010Article
- Construction and characterization of a bacterial artificial chromosome (BAC) library of Pacific white shrimp, Litopenaeus vannamei.Marine biotechnology (New York, N.Y.) · 2010Article
- A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes.Plant molecular biology · 2010Article
- Inheritance and molecular mapping of a downy mildew resistance gene, Pl (13) in cultivated sunflower (Helianthus annuus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Introgression and molecular tagging of Rf (4), a new male fertility restoration gene from wild sunflower Helianthus maximiliani L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Molecular mapping of a nuclear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Construction and characterization of two bacterial artificial chromosome libraries of Zhikong scallop, Chlamys farreri Jones et Preston, and identification of BAC clones containing the genes involved in its innate immune system.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complementary BAC and BIBAC libraries were constructed from nuclear DNA of sunflower cultivar HA 89. The BAC library, constructed with BamHI in the pECBAC1 vector, contains 107,136 clones and has an average insert size of 140 kb. The BIBAC library was constructed with HindIII in the plant-transformation-competent binary vector pCLD04541 and contains 84,864 clones, with an average insert size of 137 kb. The two libraries combined contain 192,000 clones and are equivalent to approximately 8.9 haploid genomes of sunflower (3,000 Mb/1C), and provide a greater than 99% probability of obtaining a clone of interest. The frequencies of BAC and BIBAC clones carrying chloroplast or mitochondrial DNA sequences were estimated to be 2.35 and 0.04%, respectively, and insert-empty clones were less than 0.5%. To facilitate chromosome engineering and anchor the sunflower genetic map to its chromosomes, one to three single- or low-copy RFLP markers from each linkage group of sunflower were used to design pairs of overlapping oligonucleotides (overgos). Thirty-six overgos were designed and pooled as probes to screen a subset (5.1x) of the BAC and BIBAC libraries. Of the 36 overgos, 33 (92%) gave at least one positive clone and 3 (8%) failed to hit any clone. As a result, 195 BAC and BIBAC clones representing 19 linkage groups were identified, including 76 BAC clones and 119 BIBAC clones, further verifying the genome coverage and utility of the libraries. These BAC and BIBAC libraries and linkage group-specific clones provide resources essential for comprehensive research of the sunflower genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.