ArticleNucleic acids research2006
Small protein B interacts with the large and the small subunits of a stalled ribosome during trans-translation.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- The bacterial translation stress response.FEMS microbiology reviews · 2014Review
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.Journal of nucleic acids · 2011Article
- tmRNA-SmpB: a journey to the centre of the bacterial ribosome.The EMBO journal · 2010Article
- Role of the C-terminal tail of SmpB in the early stage of trans-translation.RNA (New York, N.Y.) · 2010Article
- Ribosome hijacking: a role for small protein B during trans-translation.EMBO reports · 2009Article
- Escherichia coli tmRNA lacking pseudoknot 1 tags truncated proteins in vivo and in vitro.RNA (New York, N.Y.) · 2009Article
- The highest affinity binding site of small protein B on transfer messenger RNA is outside the tRNA domain.RNA (New York, N.Y.) · 2008Article
- Functional SmpB-ribosome interactions require tmRNA.The Journal of biological chemistry · 2007Article
- A functional interaction of SmpB with tmRNA for determination of the resuming point of trans-translation.RNA (New York, N.Y.) · 2007Article
- Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.The Journal of biological chemistry · 2007Article
- Interaction of SmpB with ribosome from directed hydroxyl radical probing.Nucleic acids research · 2007Article
- Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During trans-translation, stalled bacterial ribosomes are rescued by small protein B (SmpB) and by transfer-messenger RNA (tmRNA). Stalled ribosomes switch translation from the defective messages to a short internal reading frame on tmRNA that tags the nascent peptide chain for degradation and recycles the ribosomes. We present evidences that SmpB binds the large and small ribosomal subunits in vivo and in vitro. The binding between SmpB and the ribosomal subunits is very tight, with a dissociation constant of 1.7 x 10(-10) M, similar to its K(D) for the 70S ribosome or for tmRNA. tmRNA displaces SmpB from its 50S binding but not from the 30S. In vivo, SmpB is detected on the 50S when trans-translation is impaired by lacking tmRNA or a functional SmpB. SmpB contacts the large subunit transiently and early during the trans-translational process. The affinity of SmpB for the two ribosomal subunits is modulated by tmRNA in the course of trans-translation. It is the first example of two copies of the same protein interacting with two different functional sites of the ribosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.