ArticleElectrophoresis2006
Microscale analysis of proteins in inner ear tissues and fluids with emphasis on endolymphatic sac, otoconia, and organ of Corti.
Article in Electrophoresis, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- Poly-symptomatology of chronic multi-canalicular benign paroxysmal positional vertigo: a deductive, inductive, and abductive narrative review.Frontiers in neurology · 2025Review
- A minimal ion-chemistry model for predicting benign paroxysmal positional vertigo risk based on endolymphatic calcium and pH.Frontiers in neurology · 2025Article
- Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis.iScience · 2024Article
- Up-Regulated Expression of Interferon-Gamma, Interleukin-6 and Tumor Necrosis Factor-Alpha in the Endolymphatic Sac of Meniere's Disease Suggesting the Local Inflammatory Response Underlies the Mechanism of This Disease.Frontiers in neurology · 2022Article
- Isolation of sensory hair cell specific exosomes in human perilymph.Neuroscience letters · 2021Observational
- Otoconia Structure After Short- and Long-Duration Exposure to Altered Gravity.Journal of the Association for Research in Otolaryngology : JARO · 2021Article
- The proteome of the human endolymphatic sac endolymph.Scientific reports · 2021Article
- Otopetrin-2 Immunolocalization in the Human Macula Utricle.The Annals of otology, rhinology, and laryngology · 2019Article
- Semicircular canal biomechanics in health and disease.Journal of neurophysiology · 2019Review
- Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.Proteome science · 2018Article
- Spatiotemporal differences in otoconial gene expression.Genesis (New York, N.Y. : 2000) · 2016Article
- Mechanisms of otoconia and otolith development.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Review
- Effect of Otoconial Proteins Fetuin A, Osteopontin, and Otoconin 90 on the Nucleation and Growth of Calcite.Crystal growth & design · 2015Article
- Bottom-up and shotgun proteomics to identify a comprehensive cochlear proteome.Journal of visualized experiments : JoVE · 2014Article
- Article
- In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90.PloS one · 2014Article
- In-depth proteomic analysis of mouse cochlear sensory epithelium by mass spectrometry.Journal of proteome research · 2013Article
- Immunogold TEM of otoconin 90 and otolin - relevance to mineralization of otoconia, and pathogenesis of benign positional vertigo.Hearing research · 2012Article
- Proteomic analysis of the organ of corti using nanoscale liquid chromatography coupled with tandem mass spectrometry.International journal of molecular sciences · 2012Article
- Identification of new signaling components in the sensory epithelium of human saccule.Frontiers in neurology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe preparatory techniques adapted for the study of proteins of inner ear tissues and fluids that have allowed us to apply state-of-the-art analytical techniques in spite of the minute size and anatomical complexities of this organ. Illustrative examples address unresolved issues of functional and clinical significance. First, we demonstrate how quick-freezing and freeze drying prevents artifacts that arise from sampling endolymphatic sac (ES) content in the liquid state. This set the stage for the generation of the first protein profile of the ES. Identification of crucial proteins will help elucidate mechanisms of endolymph volume regulation and pathogenesis of Meniere's disease. Second, we show how a unique situation allowed identification of otoconial proteins by mass spectrometric analysis without prior separation and we discuss possible roles for these minor otoconins in otoconial development and prevention of degenerative diseases that affect balance. Finally, we demonstrate techniques for the precise dissection of organ of Corti and its substructures, while preserving their near normal chemical state. We extended an earlier study in which we identified a novel calcium-binding protein by IEF, oncomodulin, localized in the outer hair cells and show here the applicability of prefractionation for the screening of calcium-binding proteins of organ of Corti. These studies demonstrate how advanced preparatory and analytical techniques can be applied to studies of the inner ear.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.