ArticlePlant cell reports2006
Comparative analysis of 35S and lectin promoters in transgenic soybean tissue using an automated image acquisition system and image analysis.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Benchmarking Intrinsic Promoters and Terminators for Plant Synthetic Biology Research.Biodesign research · 2022Article
- A novel cis-acting element in the GmERF3 promoter contributes to inducible gene expression in soybean and tobacco after wounding.Plant cell reports · 2016Article
- Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator.Plant cell reports · 2011Article
- High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline.BMC plant biology · 2010Article
- Robotics and dynamic image analysis for studies of gene expression in plant tissues.Journal of visualized experiments : JoVE · 2010Article
- A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean.Plant cell reports · 2009Article
- Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system.Plant cell reports · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimensional robotics table and a programmable image acquisition system, which was used to semi-continuously monitor gfp gene expression during development of transgenic soybean [Glycine max (L.) Merrill] somatic embryos. Quantitative analysis of GFP expression showed that, during somatic embryo proliferation and early development, expression of lectin-GFP was not detected. During late embryo development, expression of lectin-GFP gradually increased until the levels were similar to those of 35S-GFP. The use of an automated image collection system and image analysis facilitated the frequent monitoring and quantification of gfp gene expression on a large number of samples over an extended period of time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.