ArticleExtremophiles : life under extreme conditions2006
Characterization of a beta-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius.
Article in Extremophiles : life under extreme conditions, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Characterization of a New Glucose-Tolerant GH1 β-Glycosidase fromInternational journal of molecular sciences · 2023Article
- Unveiling the complete genome sequence of Alicyclobacillus acidoterrestris DSM 3922T, a taint-producing strain.G3 (Bethesda, Md.) · 2022Article
- Release of Soybean Isoflavones by Using a β-Glucosidase from Alicyclobacillus herbarius.Chembiochem : a European journal of chemical biology · 2021Article
- Cellulases from Thermophiles Found by Metagenomics.Microorganisms · 2018Review
- A highly glucose-tolerant GH1 β-glucosidase with greater conversion rate of soybean isoflavones in monogastric animals.Journal of industrial microbiology & biotechnology · 2018Article
- Glycoside hydrolase gene transcription byBiotechnology for biofuels · 2018Article
- Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius.Journal of industrial microbiology & biotechnology · 2017Article
- A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals.Biotechnology for biofuels · 2016Article
- Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius.Journal of industrial microbiology & biotechnology · 2016Article
- Ecological roles of dominant and rare prokaryotes in acid mine drainage revealed by metagenomics and metatranscriptomics.The ISME journal · 2015Article
- Two promising alkaline β-glucosidases isolated by functional metagenomics from agricultural soil, including one showing high tolerance towards harsh detergents, oxidants and glucose.Journal of industrial microbiology & biotechnology · 2014Article
- Characterization of a novel β-glucosidase from a compost microbial metagenome with strong transglycosylation activity.The Journal of biological chemistry · 2013Article
- Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with β-glucosidase, β-fucosidase and β-galactosidase activities.BMC biotechnology · 2013Article
- Complete genome sequence of Alicyclobacillus acidocaldarius strain Tc-4-1.Journal of bacteriology · 2011Article
- Complete genome sequence of Alicyclobacillus acidocaldarius type strain (104-IA).Standards in genomic sciences · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In cell free extracts of the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius ATCC27009, we have identified beta-gluco- and galactosidase activities showing a specific activity of 0.1 and 12 U/mg, respectively. The two enzymatic activities are associated with different polypeptides and we show here the functional cloning, the expression in Escherichia coli and the characterisation of the beta-glucosidase (Aabeta-gly). The enzyme, which is optimally active and stable at temperatures above 65 degrees C, belongs to glycoside hydrolase family 1 (GH1) and shows wide substrate specificity on different aryl-glycosides and cello-oligosaccharides with k (cat)/K (M) for 4-nitrophenyl-beta-D-glucoside and cellobiose of 2,976 and 185 s(-1)mM(-1), respectively. Interestingly, upstream to the beta-glycosidase gene, we identified a second ORF homologous to the ATPase subunit of the bacterial ABC transporters (abc1) that is co-transcribed with the beta-glycosidase gene glyB and that could be involved in the carbohydrate import. The activity of the enzyme on cello-oligosaccharides of up to five glucose units strongly indicates that the enzyme could be involved in vivo in the degradation of glucans together with endoglucanase enzymes previously described. This, together with the co-expression of the two genes, suggests a role for the glyB-abc1 cluster in A. acidocaldarius in the degradation of cellulose and hemicelluloses.
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