ArticleBiochemistry2006
Caged vanilloid ligands for activation of TRPV1 receptors by 1- and 2-photon excitation.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Bioorthogonal Photoactivation of 2-Nitrobenzyl Caged Doxorubicin Anticancer Prodrugs on Gold Nanostars.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Light-Controlled Modulation of the Endocannabinoid System: Photoswitchable Ligands for Cannabinoid and TRPV1 Receptors.International journal of molecular sciences · 2026Review
- Photoactivatable Plant Hormone-Based Chemical Inducers of Proximity forACS chemical biology · 2025Article
- Discovery of a Novel Photocaged PI3K Inhibitor Capable of Real-Time Reporting of Drug Release.ACS medicinal chemistry letters · 2023Article
- Reverse Engineering Caged Compounds: Design Principles for their Application in Biology.Angewandte Chemie (International ed. in English) · 2023Review
- Immune aspects of the bi-directional neuroimmune facilitator TRPV1.Molecular biology reports · 2019Review
- Ncm, a Photolabile Group for Preparation of Caged Molecules: Synthesis and Biological Application.PloS one · 2016Article
- Photoactivatable, biologically-relevant phenols with sensitivity toward 2-photon excitation.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Article
- Light-activated serotonin for exploring its action in biological systems.Chemistry & biology · 2013Article
- Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy.Chemical reviews · 2013Review
- A chemist and biologist talk to each other about caged neurotransmitters.Beilstein journal of organic chemistry · 2013Article
- Optogenetic induction of aversive taste memory.Neuroscience · 2012Article
- 20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.The Journal of biological chemistry · 2012Article
- The anti-botulism triterpenoid toosendanin elicits calcium increase and exocytosis in rat sensory neurons.Cellular and molecular neurobiology · 2011Article
- Optogenetic control of cells and circuits.Annual review of cell and developmental biology · 2011Review
- The identification of a caffeine-induced Ca2+ influx pathway in rat primary sensory neurons.Molecular and cellular biochemistry · 2009Article
- Optical switches for remote and noninvasive control of cell signaling.Science (New York, N.Y.) · 2008Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Nociceptive neurons in the peripheral nervous system detect noxious stimuli and report the information to the central nervous system. Most nociceptive neurons express the vanilloid receptor, TRPV1, a nonselective cation channel gated by vanilloid ligands such as capsaicin, the pungent essence of chili peppers. Here, we report the synthesis and biological application of two caged vanilloids: biologically inert precursors that, when photolyzed, release bioactive vanilloid ligands. The two caged vanilloids, Nb-VNA and Nv-VNA, are photoreleased with quantum efficiency of 0.13 and 0.041, respectively. Under flash photolysis conditions, photorelease of Nb-VNA and Nv-VNA is 95% complete in approximately 40 micros and approximately 125 micros, respectively. Through 1-photon excitation with ultraviolet light (360 nm), or 2-photon excitation with red light (720 nm), the caged vanilloids can be photoreleased in situ to activate TRPV1 receptors on nociceptive neurons. The consequent increase in intracellular free Ca(2+) concentration ([Ca(2+)](i)) can be visualized by laser-scanning confocal imaging of neurons loaded with the fluorescent Ca(2+) indicator, fluo-3. Stimulation results from TRPV1 receptor activation, because the response is blocked by capsazepine, a selective TRPV1 antagonist. In Ca(2+)-free extracellular medium, photoreleased vanilloid can still elevate [Ca(2+)](i), which suggests that TRPV1 receptors also reside on endomembranes in neurons and can mediate Ca(2+) release from intracellular stores. Notably, whole-cell voltage clamp measurements showed that flash photorelease of vanilloid can activate TRPV1 channels in <4 ms at 22 degrees C. In combination with 1- or 2-photon excitation, caged vanilloids are a powerful tool for probing morphologically distinct structures of nociceptive sensory neurons with high spatial and temporal precision.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.