ArticleThe Plant cell2006
Gamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 98 citations in OpenAlex.
- Stress-induced MAPK (SIMK)-dependent organization of microtubules in alfalfa.Molecular horticulture · 2026Article
- Genome-wide identification, characterization and expression analysis of tubulin gene family in Populus deltoides.BMC plant biology · 2025Article
- A procedure for Dex-induced gene transactivation in Arabidopsis ovules.Plant methods · 2022Article
- Global gene expression profiling under nitrogen stress identifies key genes involved in nitrogen stress adaptation in maize (Zea mays L.).Scientific reports · 2022Article
- γ-Tubulin in microtubule nucleation and beyond.Frontiers in cell and developmental biology · 2022Review
- Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366.BMC genomics · 2021Article
- Article
- γ-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions.Cells · 2021Review
- Choreography of the centrosome.Heliyon · 2020Review
- The nuclear envelope in higher plant mitosis and meiosis.Nucleus (Austin, Tex.) · 2019Review
- Review
- Expression of both Arabidopsis γ-tubulin genes is essential for development of a functional syncytium induced by Heterodera schachtii.Plant cell reports · 2018Article
- Article
- Review
- Structure, function, and evolution of plant NIMA-related kinases: implication for phosphorylation-dependent microtubule regulation.Journal of plant research · 2015Review
- The functions of the cytoskeleton and associated proteins during mitosis and cytokinesis in plant cells.Frontiers in plant science · 2015Review
- Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.Plant physiology · 2014Article
- MAPs: cellular navigators for microtubule array orientations in Arabidopsis.Plant cell reports · 2014Review
- Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells.Journal of experimental botany · 2013Article
- Interactions of an Arabidopsis RanBPM homologue with LisH-CTLH domain proteins revealed high conservation of CTLH complexes in eukaryotes.BMC plant biology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Gamma-tubulin is required for microtubule (MT) nucleation at MT organizing centers such as centrosomes or spindle pole bodies, but little is known about its noncentrosomal functions. We conditionally downregulated gamma-tubulin by inducible expression of RNA interference (RNAi) constructs in Arabidopsis thaliana. Almost complete RNAi depletion of gamma-tubulin led to the absence of MTs and was lethal at the cotyledon stage. After induction of RNAi expression, gamma-tubulin was gradually depleted from both cytoplasmic and microsomal fractions. In RNAi plants with partial loss of gamma-tubulin, MT recovery after drug-induced depolymerization was impaired. Similarly, immunodepletion of gamma-tubulin from Arabidopsis extracts severely compromised in vitro polymerization of MTs. Reduction of gamma-tubulin protein levels led to randomization and bundling of cortical MTs. This finding indicates that MT-bound gamma-tubulin is part of a cortical template guiding the microtubular network and is essential for MT nucleation. Furthermore, we found that cells with decreased levels of gamma-tubulin could progress through mitosis, but cytokinesis was strongly affected. Stepwise diminution of gamma-tubulin allowed us to reveal roles for MT nucleation in plant development, such as organization of cell files, anisotropic and polar tip growth, and stomatal patterning. Some of these functions of gamma-tubulin might be independent of MT nucleation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.