ArticleBiophysical journal2006
Diffusional anisotropy in collagenous tissues: fluorescence imaging of continuous point photobleaching.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 85 citations in OpenAlex.
- Establishment of preparation methods foriScience · 2026Article
- Key roles of the superficial zone in articular cartilage physiology, pathology, and regeneration.Chinese medical journal · 2025Review
- Assessing the role of surface layer and molecular probe size in diffusion within meniscus tissue.PloS one · 2024Article
- A Comparative Analysis of Orthotopic and Subcutaneous Pancreatic Tumour Models: Tumour Microenvironment and Drug Delivery.Cancers · 2023Article
- [Study on transport of small molecule rhodamine B within different layers of cartilage].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2022Article
- Diffusion Tensor and Dynamic Contrast-Enhanced Magnetic Resonance Imaging Correlate with Molecular Markers of Inflammation in the Synovium.Diagnostics (Basel, Switzerland) · 2022Article
- Intra-articular drug delivery systems for osteoarthritis therapy: shifting from sustained release to enhancing penetration into cartilage.Drug delivery · 2022Review
- Strain-Dependent Diffusivity of Small and Large Molecules in Meniscus.Journal of biomechanical engineering · 2022Article
- Contrast solution properties and scan parameters influence the apparent diffusivity of computed tomography contrast agents in articular cartilage.Journal of the Royal Society, Interface · 2022Article
- An Additively Manufactured Sample Holder to Measure the Controlled Release of Vancomycin from Collagen Laminates.Biomedicines · 2021Article
- Size-Dependent Effective Diffusivity in Healthy Human and Porcine Joint Synovium.Annals of biomedical engineering · 2021Article
- A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion.Nature communications · 2021Article
- An MRI study of solute transport in the intervertebral disc.Magma (New York, N.Y.) · 2020Article
- Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.Osteoarthritis and cartilage · 2020Article
- Molecular transport in articular cartilage - what have we learned from the past 50 years?Nature reviews. Rheumatology · 2018Review
- Tailoring drug release rates in hydrogel-based therapeutic delivery applications using graphene oxide.Journal of the Royal Society, Interface · 2018Article
- Direct Quantification of Solute Diffusivity in Agarose and Articular Cartilage Using Correlation Spectroscopy.Annals of biomedical engineering · 2017Article
- Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport.Tissue engineering. Part B, Reviews · 2014Review
- Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching.Annals of biomedical engineering · 2014Article
- Effects of cartilage impact with and without fracture on chondrocyte viability and the release of inflammatory markers.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular transport in avascular collagenous tissues such as articular cartilage occurs primarily via diffusion. The presence of ordered structures in the extracellular matrix may influence the local transport of macromolecules, leading to anisotropic diffusion depending on the relative size of the molecule and that of extracellular matrix structures. Here we present what we believe is a novel photobleaching technique for measuring the anisotropic diffusivity of macromolecules in collagenous tissues. We hypothesized that macromolecular diffusion is anisotropic in collagenous tissues, depending on molecular size and the local organization of the collagen structure. A theoretical model and experimental protocol for fluorescence imaging of continuous point photobleaching was developed to measure diffusional anisotropy. Significant anisotropy was observed in highly ordered collagenous tissues such as ligament, with diffusivity ratios>2 along the fiber direction compared to the perpendicular direction. In less-ordered tissues such as articular cartilage, diffusional anisotropy was dependent on site in the tissue and size of the diffusing molecule. Anisotropic diffusion was also dependent on the size of the diffusing molecule, with greatest anisotropy observed for larger molecules. These findings suggest that diffusional transport of macromolecules is anisotropic in collagenous tissues, with higher rates of diffusion along primary orientation of collagen fibers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.