ArticleBiophysical journal2006
Lateral diffusion anisotropy and membrane lipid/skeleton interaction in outer hair cells.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Auditory Hair Cell Mechanotransduction Channels Dynamically Shape the Mechanical Properties of Their Membrane Environment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Radixin modulates the function of outer hair cell stereocilia.Communications biology · 2020Article
- Rat Auditory Inner Hair Cell Mechanotransduction and Stereociliary Membrane Diffusivity Are Similarly Modulated by Calcium.iScience · 2020Article
- Spectrin βV adaptive mutations and changes in subcellular location correlate with emergence of hair cell electromotility in mammalians.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins.PLoS genetics · 2015Article
- Unconventional mechanics of lipid membranes: a potential role for mechanotransduction of hair cell stereocilia.Biophysical journal · 2015Article
- Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction.Plant cell reports · 2013Article
- Microdomains shift and rotate in the lateral wall of cochlear outer hair cells.Biophysical journal · 2013Article
- Intracellular Delivery of Short Interfering RNA in Rat Organ of Corti Using a Cell-penetrating Peptide PepFect6.Molecular therapy. Nucleic acids · 2012Article
- Membrane cholesterol strongly influences confined diffusion of prestin.Biophysical journal · 2012Article
- Sound-induced length changes in outer hair cell stereocilia.Nature communications · 2012Article
- Prestin in HEK cells is an obligate tetramer.Journal of neurophysiology · 2012Article
- Atomistic simulation of lipid and DiI dynamics in membrane bilayers under tension.Physical chemistry chemical physics : PCCP · 2011Article
- Voltage and frequency dependence of prestin-associated charge transfer.Journal of theoretical biology · 2009Article
- Lipid lateral mobility in cochlear outer hair cells: regional differences and regulation by cholesterol.Journal of the Association for Research in Otolaryngology : JARO · 2009Article
- Diffusion in a fluid membrane with a flexible cortical cytoskeleton.Biophysical journal · 2009Article
- Immune atomic force microscopy of prestin-transfected CHO cells using quantum dots.Pflugers Archiv : European journal of physiology · 2009Article
- Amphipath-induced nanoscale changes in outer hair cell plasma membrane curvature.Biophysical journal · 2009Article
- A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy.Biophysical journal · 2008Article
- Tuning of the outer hair cell motor by membrane cholesterol.The Journal of biological chemistry · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The organization of the plasma membrane of cells in lipid domains affects the way the membrane interacts with the underlying protein skeleton, which in turn affects the lateral mobility of lipid and protein molecules in the membrane. Membrane fluidity properties can be monitored by various approaches, the most versatile of which is fluorescence recovery after photobleaching (FRAP). We extended previous FRAP experiments on isolated cochlear outer hair cells (OHCs) by analyzing the two-dimensional pattern of lipid diffusion in the lateral membrane of these cells. We found that membrane lipid mobility in freshly isolated OHCs is orthotropic, diffusion being faster in the axial direction of the cell and slower in the circumferential direction. Increasing the cell's turgor pressure by osmotic challenge reduced the axial diffusion constant, but had only a slight effect on circumferential diffusion. Our results suggest that lipid mobility in the OHC plasma membrane is affected by the presence of the cell's orthotropic membrane skeleton. This effect could reflect interaction with spectrin filaments or with other membrane skeletal proteins. We also performed a number of FRAP measurements in temporal bone preparations preserving the structural integrity of the hearing organ. The diffusion rates measured for OHCs in this preparation were in good agreement with those obtained in isolated OHCs, and comparable to the mobility rates measured on the sensory inner hair cells. These observations support the idea that the plasma membranes of both types of hair cells share similar highly fluid phases in the intact organ. Lipid mobility was significantly slower in the membranes of supporting cells of the organ of Corti, which could reflect differences in lipid phase or stronger hindrance by the cytoskeleton in these membranes.
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