ArticleBiophysical journal2006
The DNA binding activity of p53 displays reaction-diffusion kinetics.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 80 citations in OpenAlex.
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- Transcription Factor Dynamics.Cold Spring Harbor perspectives in biology · 2021Review
- Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion.Journal of mathematical biology · 2021Article
- Linker DNA and histone contributions in nucleosome binding by p53.Journal of biochemistry · 2020Article
- Diffusion of DNA-Binding Species in the Nucleus: A Transient Anomalous Subdiffusion Model.Biophysical journal · 2020Article
- Quantitating repair protein accumulation at DNA lesions: Past, present, and future.DNA repair · 2019Review
- p53 pulse modulation differentially regulates target gene promoters to regulate cell fate decisions.Molecular systems biology · 2019Article
- Defining TP53 pioneering capabilities with competitive nucleosome binding assays.Genome research · 2019Article
- Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.Chemical reviews · 2018Review
- Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity.Nature communications · 2017Article
- Improving Parameter Inference from FRAP Data: an Analysis Motivated by Pattern Formation in the Drosophila Wing Disc.Bulletin of mathematical biology · 2017Article
- The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis.PloS one · 2017Article
- Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos.BMC biology · 2016Review
- Reaction-diffusion systems for spatio-temporal intracellular protein networks: A beginner's guide with two examples.Computational and structural biotechnology journal · 2014Review
- Spatial invasion dynamics on random and unstructured meshes: implications for heterogeneous tumor populations.Journal of theoretical biology · 2014Article
- Convergence of chromatin binding estimates in live cells.Nature methods · 2013Article
- Parameter importance in FRAP acquisition and analysis: a simulation approach.Biophysical journal · 2013Article
- Article
- Analysis of protein and lipid dynamics using confocal fluorescence recovery after photobleaching (FRAP).Current protocols in cytometry · 2012Article
- Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.Biophysical journal · 2012Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The tumor suppressor protein p53 plays a key role in maintaining the genomic stability of mammalian cells and preventing malignant transformation. In this study, we investigated the intracellular diffusion of a p53-GFP fusion protein using confocal fluorescence recovery after photobleaching. We show that the diffusion of p53-GFP within the nucleus is well described by a mathematical model for diffusion of particles that bind temporarily to a spatially homogeneous immobile structure with binding and release rates k1 and k2, respectively. The diffusion constant of p53-GFP was estimated to be Dp53-GFP=15.4 microm2 s-1, significantly slower than that of GFP alone, DGFP=41.6 microm2 s-1. The reaction rates of the binding and unbinding of p53-GFP were estimated as k1=0.3 s-1 and k2=0.4 s-1, respectively, values suggestive of nonspecific binding. Consistent with this finding, the diffusional mobilities of tumor-derived sequence-specific DNA binding mutants of p53 were indistinguishable from that of the wild-type protein. These data are consistent with a model in which, under steady-state conditions, p53 is latent and continuously scans DNA, requiring activation for sequence-specific DNA binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.