ArticleHistochemistry and cell biology2006
Histochemical analyses of living mouse liver under different hemodynamic conditions by "in vivo cryotechnique".
Article in Histochemistry and cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- Proximal tubule morphology in rats with renal congestion: a study involving the in vivo cryotechnique.Medical molecular morphology · 2015Article
- Immunohistochemical and morphofunctional studies of skeletal muscle tissues with electric nerve stimulation by in vivo cryotechnique.Acta histochemica et cytochemica · 2015Article
- Immuno- and Enzyme-histochemistry of HRP for Demonstration of Blood Vessel Permeability in Mouse Thymic Tissues by "In Vivo Cryotechnique".Acta histochemica et cytochemica · 2014Article
- Immunohistochemical detection of angiotensin II receptors in mouse cerebellum and adrenal gland using "in vivo cryotechnique".Histochemistry and cell biology · 2013Article
- Detection of MAPK signal transduction proteins in an ischemia/reperfusion model of mouse intestine using in vivo cryotechnique.Histochemistry and cell biology · 2013Article
- Immunohistochemical analysis of various serum proteins in living mouse thymus with "in vivo cryotechnique".Medical molecular morphology · 2012Article
- Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues by in vivo cryotechnique and cryobiopsy.Medical molecular morphology · 2011Article
- Histological study and LYVE-1 immunolocalization of mouse mesenteric lymph nodes with "In Vivo Cryotechnique".Acta histochemica et cytochemica · 2011Article
- Immunohistochemical distribution of serum proteins in living mouse heart with In vivo cryotechnique.Acta histochemica et cytochemica · 2011Article
- Histochemical approach of cryobiopsy for glycogen distribution in living mouse livers under fasting and local circulation loss conditions.Histochemistry and cell biology · 2010Article
- Immunoreactivity of glutamate in mouse retina inner segment of photoreceptors with in vivo cryotechnique.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Recent progress in histochemistry.Histochemistry and cell biology · 2007Review
- Immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole using "in vivo cryotechnique".Histochemistry and cell biology · 2007Article
- The histochemistry and cell biology vade mecum: a review of 2005-2006.Histochemistry and cell biology · 2006Review
- Morphological analysis of lamellar structures in mouse type II pneumocytes by quick-freezing and freeze-drying with osmium tetroxide vapor-fixation.Medical molecular morphology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the morphology and molecular distribution in animal liver tissues have been examined using conventional preparation methods, the findings are always affected by the technical artifacts caused by perfusion-fixation and tissue-resection. Using "in vivo cryotechnique" (IVCT), we have examined living mouse livers with histochemical, immunohistochemical and ultrastructural analyses. In samples prepared by IVCT, widely open sinusoids with many flowing erythrocytes were observed under normal blood circulation, and their collapse or blood congestion was seen in ischemic or heart-arrested mice. In contrast, the sinusoidal cavities were artificially dilated by perfusion-fixation, and collapsed by immersion-fixation and quick-freezing (QF) methods of resected tissues. The immunoreactivity of serum albumin and immunoglobulin G and intensity of periodic acid-Schiff-staining in hepatocytes were well preserved with the QF method and IVCT. Furthermore, following tissue resection, serum proteins were rapidly translocated into hepatocytes as demonstrated by immunoreactions on QF tissues frozen 1 or 5 min after resection. Translocation was not observed in IVCT samples, indicating that IVCT could be useful to examine cell membrane permeability of hepatocytes under different pathological conditions. Both dynamic morphology and immunodistribution of soluble components in living mouse livers, reflecting their physiological and pathological states, can be precisely examined by IVCT with higher time-resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.