ArticleThe EMBO journal2006
GGA function is required for maturation of neuroendocrine secretory granules.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Arf GTPase-activating proteins SMAP1 and AGFG2 regulate the size of Weibel-Palade bodies and exocytosis of von Willebrand factor.Biology open · 2021Article
- Cdc42 controls secretory granules morphology in rodent salivary glands in vivo.Communicative & integrative biology · 2020Article
- Induction of membrane curvature by proteins involved in Golgi trafficking.Advances in biological regulation · 2020Review
- HID-1 controls formation of large dense core vesicles by influencing cargo sorting andMolecular biology of the cell · 2017Article
- Role of clathrin in dense core vesicle biogenesis.Molecular biology of the cell · 2017Article
- The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles.PLoS genetics · 2016Article
- Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.PLoS genetics · 2014Article
- Article
- Rab40b regulates trafficking of MMP2 and MMP9 during invadopodia formation and invasion of breast cancer cells.Journal of cell science · 2013Article
- Bioanalysis of eukaryotic organelles.Chemical reviews · 2013Review
- Role of adaptor proteins in secretory granule biogenesis and maturation.Frontiers in endocrinology · 2013Article
- Article
- Cholesterol accumulation increases insulin granule size and impairs membrane trafficking.Traffic (Copenhagen, Denmark) · 2012Article
- Drosophila GGA model: an ultimate gateway to GGA analysis.Traffic (Copenhagen, Denmark) · 2011Article
- HID-1, a new component of the peptidergic signaling pathway.Genetics · 2011Article
- Roles of myosin Va and Rab3D in membrane remodeling of immature secretory granules.Cellular and molecular neurobiology · 2010Article
- Lamellipodium extension and membrane ruffling require different SNARE-mediated trafficking pathways.BMC cell biology · 2010Article
- Effect of methylene blue on the genomic response to reperfusion injury induced by cardiac arrest and cardiopulmonary resuscitation in porcine brain.BMC medical genomics · 2010Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory granule (SG) maturation has been proposed to involve formation of clathrin-coated vesicles (CCVs) from immature SGs (ISGs). We tested the effect of inhibiting CCV budding by using the clathrin adaptor GGA (Golgi-associated, gamma-ear-containing, ADP-ribosylation factor-binding protein) on SG maturation in neuroendocrine cells. Overexpression of a truncated, GFP-tagged GGA, VHS (Vps27, Hrs, Stam)-GAT (GGA and target of myb (TOM))-GFP led to retention of MPR, VAMP4, and syntaxin 6 in mature SGs (MSGs), suggesting that CCV budding from ISGs is inhibited by the SG-localizing VHS-GAT-GFP. Furthermore, VHS-GAT-GFP-overexpression disrupts prohormone convertase 2 (PC2) autocatalytic cleavage, processing of secretogranin II to its product p18, and the correlation between PC2 and p18 levels. All these effects were not observed if full-length GGA1-GFP was overexpressed. Neither GGA1-GFP nor VHS-GAT-GFP perturbed SG protein budding from the TGN, or homotypic fusion of ISGs. Reducing GGA3 levels by using short interfering (si)RNA also led to VAMP4 retention in SGs, and inhibition of PC2 activity. Our results suggest that inhibition of CCV budding from ISGs downregulates the sorting from the ISGs and perturbs the intragranular activity of PC2.
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