ArticleProceedings of the National Academy of Sciences of the United States of America1991
DNA binding by the archaeal histone HMf results in positive supercoiling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 111 citations in OpenAlex.
- Regulation of DNA Topology in Archaea: State of the Art and Perspectives.Molecular microbiology · 2025Review
- SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo.Scientific reports · 2025Article
- The regulation of DNA supercoiling across evolution.Protein science : a publication of the Protein Society · 2021Review
- Mechanical and structural properties of archaeal hypernucleosomes.Nucleic acids research · 2021Article
- Archaea: The Final Frontier of Chromatin.Journal of molecular biology · 2021Review
- Chromatin structure and dynamics in hot environments: architectural proteins and DNA topoisomerases of thermophilic archaea.International journal of molecular sciences · 2014Review
- Review
- Centromere identity: a challenge to be faced.Molecular genetics and genomics : MGG · 2010Review
- Twisting of the DNA-binding surface by a beta-strand-bearing proline modulates DNA gyrase activity.Nucleic acids research · 2010Article
- In the absence of writhe, DNA relieves torsional stress with localized, sequence-dependent structural failure to preserve B-form.Nucleic acids research · 2009Article
- Article
- An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA.BioTechniques · 2009Article
- Nanoarchaeal origin of histone H3?Journal of bacteriology · 2009Article
- Archaeal chromatin proteins histone HMtB and Alba have lost DNA-binding ability in laboratory strains of Methanothermobacter thermautotrophicus.Extremophiles : life under extreme conditions · 2008Article
- DNA protection by histone-like protein HU from the hyperthermophilic eubacterium Thermotoga maritima.Nucleic acids research · 2008Article
- TrpY regulation of trpB2 transcription in Methanothermobacter thermautotrophicus.Journal of bacteriology · 2008Article
- Biochemical characterization of DNA-binding proteins from Pyrobaculum aerophilum and Aeropyrum pernix.Extremophiles : life under extreme conditions · 2008Article
- Histones in crenarchaea.Journal of bacteriology · 2005Article
- Functional domains of the ubiquitous chromatin protein DEK.Molecular and cellular biology · 2004Article
- Conserved eukaryotic histone-fold residues substituted into an archaeal histone increase DNA affinity but reduce complex flexibility.Journal of bacteriology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HMf, a histone from the hyperthermophilic archaeon Methanothermus fervidus binds double-stranded DNA molecules in vitro, forming compact structures that visibly resemble eukaryal nucleosomes. We show here that HMf binding increases the helical periodicity of DNA molecules to approximately 11 base pairs (bp) per turn and that DNA molecules in these nucleosome-like structures are constrained in positive toroidal supercoils. Based on the mass of HMf needed to cause a change in linking number (delta Lk), the maximum delta Lk introduced into circular DNA molecules of known sizes, and electron microscopy, we estimate that each HMf-DNA structure contains between 90 and 150 bp of DNA wrapped in 1.5 positive toroidal supercoils around a core of four HMf molecules. A model and pathway for the formation of these structures in vitro are presented and the possible role of positive toroidal wrapping of the M. fervidus genome in vivo is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.