ArticlePflugers Archiv : European journal of physiology1991
Calcium- and cyclic-AMP-mediated secretory responses in isolated colonic crypts.
Article in Pflugers Archiv : European journal of physiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 109 citations in OpenAlex.
- Time-dependent effects of tumor necrosis factor α on CaFrontiers in physiology · 2024Article
- Impact of Sensitization and Inflammation on the Interaction of Mast Cells With the Intestinal Epithelium in Rats.Frontiers in physiology · 2019Article
- Hypoxia/Reoxygenation Effects on Ion Transport across Rat Colonic Epithelium.Frontiers in physiology · 2016Article
- STIM1-regulated Ca2+ influx across the apical and the basolateral membrane in colonic epithelium.The Journal of membrane biology · 2013Article
- Modulation of ion transport across rat distal colon by cysteine.Frontiers in physiology · 2012Article
- Sites of action of hydrogen peroxide on ion transport across rat distal colon.British journal of pharmacology · 2008Article
- Ca2+-induced Cl- efflux at rat distal colonic epithelium.The Journal of membrane biology · 2008Article
- Cholinergic regulation of epithelial ion transport in the mammalian intestine.British journal of pharmacology · 2006Review
- Muscarinic receptor stimulation activates a Ca(2+)-dependent Cl(-) conductance in rat distal colon.The Journal of membrane biology · 2005Article
- Stimulation of colonic anion secretion by monochloramine: action sites.Pflugers Archiv : European journal of physiology · 2005Article
- Regulatory volume increase after secretory volume decrease in colonic epithelial cells under muscarinic stimulation.Pflugers Archiv : European journal of physiology · 2004Article
- Effects of dopamine on ion transport across the rat distal colon.Pflugers Archiv : European journal of physiology · 2004Article
- Activation of apical K+ conductances by muscarinic receptor stimulation in rat distal colon: fast and slow components.The Journal of membrane biology · 2003Article
- Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts.Biological procedures online · 2002Article
- 17beta-oestradiol acutely regulates Cl- secretion in rat distal colonic epithelium.The Journal of physiology · 2001Article
- Multiple action sites of flufenamate on ion transport across the rat distal colon.British journal of pharmacology · 2000Article
- Basolateral K+ channel involvement in forskolin-activated chloride secretion in human colon.The Journal of physiology · 1999Article
- Proceedings of the scientific meetings of the Physiology Society. November 1996 and January 1997. Abstracts.The Journal of physiology · 1997Article
- Cyclic AMP-dependent regulation of K+ transport in the rat distal colon.British journal of pharmacology · 1996Article
- Segmental heterogeneity of swelling-induced Cl- transport in rat small intestine.Pflugers Archiv : European journal of physiology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-cell recordings were performed at isolated crypts from the distal colon of the rat. Enterocytes in intact crypts, patched from the basolateral side, exhibited a gradient in the resting zero-current potential. Along the axis of the crypt, the highest potentials were measured in the ground region, the lowest in the surface region. The cholinergic agonist, carbachol, induced a hyperpolarization and an increase of the outward current in both the middle and the ground cells of intact crypts. This effect could be prevented by Ba2+ or by the intracellular Ca2+ antagonist, 8-(N, N-diethylamino)-octyl-3,4,5-trimethoxy-benzoate hydrochloride (TMB-8). Its action, however, was not dependent on the presence of external Ca2+. Both ground cells and the cells in the middle part of the crypt responded to forskolin, an activator of the adenylate cyclase, with a depolarization. In the middle part of the crypt, the depolarization induced by forskolin was associated with an increase of the outward current. It could be blocked by the Cl- channel blocker, 5-nitro-2-(3-phenylpropylamino)-benzoate, indicating an increase of Cl- conductance. In contrast, the forskolin-induced depolarization in the ground part of the crypt was associated with a decrease of the outward current. This effect could be prevented by Ba2+, indicating a decrease of a potassium conductance. The changes in outward current could be prevented by the presence of an inhibitor of protein kinase A in the pipette solution. In conclusion, these results suggest that carbachol, an agonist acting on the Ca2+ pathway, indirectly causes Cl- secretion by an increase of the driving force, i.e. the membrane potential. Only the activation of cyclic AMP synthesis by forskolin is able to increase Cl- conductance in the rat colon. The latter response seems to be dependent on the state of differentiation of the enterocytes.
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