ArticleOsteoarthritis and cartilage2006
Expression and cellular localization of human hyaluronidase-2 in articular chondrocytes and cultured cell lines.
Article in Osteoarthritis and cartilage, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Mechanobiology of Hyaluronan: Connecting Biomechanics and Bioactivity in Musculoskeletal Tissues.Annual review of biomedical engineering · 2024Review
- The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival.PLoS genetics · 2022Article
- Matters of size: Roles of hyaluronan in CNS aging and disease.Ageing research reviews · 2021Review
- The cell surface hyaluronidase TMEM2 is essential for systemic hyaluronan catabolism and turnover.The Journal of biological chemistry · 2021Article
- The pericellular hyaluronan of articular chondrocytes.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- TMEM2: A missing link in hyaluronan catabolism identified?Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- A mammalian homolog of the zebrafish transmembrane protein 2 (TMEM2) is the long-sought-after cell-surface hyaluronidase.The Journal of biological chemistry · 2017Article
- Hyaluronidase 2 (HYAL2) is expressed in endothelial cells, as well as some specialized epithelial cells, and is required for normal hyaluronan catabolism.Histochemistry and cell biology · 2016Article
- CD44 knock-down in bovine and human chondrocytes results in release of bound HYAL2.Matrix biology : journal of the International Society for Matrix Biology · 2015Article
- Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes.Stem cells international · 2015Article
- Hyaluronan, a crucial regulator of inflammation.Frontiers in immunology · 2014Review
- Effects of Payena dasyphylla (Miq.) on hyaluronidase enzyme activity and metalloproteinases protein expressions in interleukin-1β stimulated human chondrocytes cells.BMC complementary and alternative medicine · 2013Article
- Murine hyaluronidase 2 deficiency results in extracellular hyaluronan accumulation and severe cardiopulmonary dysfunction.The Journal of biological chemistry · 2013Article
- Reactive oxygen species and hyaluronidase 2 regulate airway epithelial hyaluronan fragmentation.The Journal of biological chemistry · 2010Article
- Chondrocyte innate immune myeloid differentiation factor 88-dependent signaling drives procatabolic effects of the endogenous Toll-like receptor 2/Toll-like receptor 4 ligands low molecular weight hyaluronan and high mobility group box chromosomal protein 1 in mice.Arthritis and rheumatism · 2010Article
- Two novel functions of hyaluronidase-2 (Hyal2) are formation of the glycocalyx and control of CD44-ERM interactions.The Journal of biological chemistry · 2009Article
- Influence of three-dimensional hyaluronic acid microenvironments on mesenchymal stem cell chondrogenesis.Tissue engineering. Part A · 2009Article
- Hyaluronidase expression and activity is regulated by pro-inflammatory cytokines in human airway epithelial cells.American journal of respiratory cell and molecular biology · 2008Article
- Differential behavior of auricular and articular chondrocytes in hyaluronic acid hydrogels.Tissue engineering. Part A · 2008Article
- Human hyaluronidase-2 is localized intracellularly in articular chondrocytes and other cultured cell lines.Osteoarthritis and cartilage · 2006Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThere is debate whether hyaluronan (HA) can be enzymatically degraded within the extracellular matrix of cartilage and other tissues or whether its catabolism occurs strictly within the lysosomal compartment of chondrocytes and other cell types. Previous studies have suggested that one of the lysosomal hyaluronidases (hyaluronidase-2) can be expressed as a functionally-active glycosyl phosphatidylinositol-linked protein at the surface of mammalian cells. If this form of hyaluronidase expression occurs in chondrocytes, this could represent a possible mechanism for extracellular HA cleavage. Thus, which hyaluronidases are expressed and where was the objective of this study.
methodsmRNA for hyaluronidases was quantified by reverse transcription-polymerase chain reaction (RT-PCR) and enzymatic activity by HA zymograms. Recombinant forms of hyaluronidase-2 were generated and expressed in model cell lines. A peptide-specific polyclonal antiserum was prepared to localize endogenous human hyaluronidase-2 in human articular chondrocytes.
resultsHyaluronidase-2 is the principal mRNA transcript expressed by primary human articular chondrocytes as well as various model cell lines. Recombinant hyaluronidase-2, containing N-terminal or C-terminal epitope tags, was strictly localized intracellularly and not released by treatment with a phosphatidylinositol-specific phospholipase. Endogenous hyaluronidase-2 expressed by human chondrocytes as well as HeLa cells could only be detected following detergent permeabilization of the plasma membranes.
conclusionsThese data suggest that on chondrocytes and other cell types examined, hyaluronidase-2 is not present or functional at the external plasma membrane. Thus, local turnover of HA is dependent on receptor-mediated endocytosis and delivery to low pH intracellular organelles for its complete degradation.
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