ArticleBMC genomics2006
Global gene expression analyses of hematopoietic stem cell-like cell lines with inducible Lhx2 expression.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Atp8a1 deletion increases the proliferative activity of hematopoietic stem cells by impairing PTEN function.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- Vitamin D Induces Global Gene Transcription in Human Corneal Epithelial Cells: Implications for Corneal Inflammation.Investigative ophthalmology & visual science · 2016Article
- Estrogen Receptor β2 Induces Hypoxia Signature of Gene Expression by Stabilizing HIF-1α in Prostate Cancer.PloS one · 2015Article
- The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Coexposure to phytoestrogens and bisphenol a mimics estrogenic effects in an additive manner.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Studies in experimental autoimmune encephalomyelitis do not support developmental bisphenol a exposure as an environmental factor in increasing multiple sclerosis risk.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Estrogen receptors β1 and β2 have opposing roles in regulating proliferation and bone metastasis genes in the prostate cancer cell line PC3.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Lhx2 is required for patterning and expansion of a distinct progenitor cell population committed to eye development.PloS one · 2011Article
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- Gene expression in murine mammary epithelial stem cell-like cells shows similarities to human breast cancer gene expression.Breast cancer research : BCR · 2009Article
- Article
- A genome-wide study of the repressive effects of estrogen receptor beta on estrogen receptor alpha signaling in breast cancer cells.Oncogene · 2008Article
- Distinctive gene expression signatures in rheumatoid arthritis synovial tissue fibroblast cells: correlates with disease activity.Genes and immunity · 2007Article
- Identification of novel stem cell markers using gap analysis of gene expression data.Genome biology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExpression of the LIM-homeobox gene Lhx2 in murine hematopoietic cells allows for the generation of hematopoietic stem cell (HSC)-like cell lines. To address the molecular basis of Lhx2 function, we generated HSC-like cell lines where Lhx2 expression is regulated by a tet-on system and hence dependent on the presence of doxycyclin (dox). These cell lines efficiently down-regulate Lhx2 expression upon dox withdrawal leading to a rapid differentiation into various myeloid cell types.
resultsGlobal gene expression of these cell lines cultured in dox was compared to different time points after dox withdrawal using microarray technology. We identified 267 differentially expressed genes. The majority of the genes overlapping with HSC-specific databases were those down-regulated after turning off Lhx2 expression and a majority of the genes overlapping with those defined as late progenitor-specific genes were the up-regulated genes, suggesting that these cell lines represent a relevant model system for normal HSCs also at the level of global gene expression. Moreover, in situ hybridisations of several genes down-regulated after dox withdrawal showed overlapping expression patterns with Lhx2 in various tissues during embryonic development.
conclusionGlobal gene expression analysis of HSC-like cell lines with inducible Lhx2 expression has identified genes putatively linked to self-renewal/differentiation of HSCs, and function of Lhx2 in organ development and stem/progenitor cells of non-hematopoietic origin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.