ArticlePlant methods2006
A novel fluorescent pH probe for expression in plants.
Article in Plant methods, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Root hair growth from the pH point of view.Frontiers in plant science · 2022Pooled it
- Linking cellular metabolism to mitochondrial outer membrane potential.Mitochondrion · 2026Article
- Reciprocal co-regulation of nitrate and ammonium transporters is modulated by external pH in Arabidopsis.Journal of experimental botany · 2026Article
- Article
- Salt-Induced Early Changes in Photosynthesis Activity Caused by Root-to-Shoot Signaling in Potato.International journal of molecular sciences · 2024Article
- Cytosolic alkalinization in guard cells: an intriguing but interesting event during stomatal closure that merits further validation of its importance.Frontiers in plant science · 2024Review
- The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (Plants (Basel, Switzerland) · 2023Article
- Salinity-Induced Cytosolic Alkaline Shifts in Arabidopsis Roots Require the SOS Pathway.International journal of molecular sciences · 2023Article
- Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.Frontiers in pharmacology · 2023Review
- Salt-Induced Changes in Cytosolic pH and Photosynthesis in Tobacco and Potato Leaves.International journal of molecular sciences · 2022Article
- Cell-Type-Specific Length and Cytosolic pH Response of Superficial Cells ofPlants (Basel, Switzerland) · 2022Article
- Biological and Molecular Components for Genetically Engineering Biosensors in Plants.Biodesign research · 2022Article
- pH biosensing in the plant apoplast-a focus on root cell elongation.Plant physiology · 2021Review
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- Multiparameter in vivo imaging in plants using genetically encoded fluorescent indicator multiplexing.Plant physiology · 2021Review
- Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality.Horticulture research · 2021Article
- Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications.Chemical reviews · 2020Article
- Pathogen-induced pH changes regulate the growth-defense balance in plants.The EMBO journal · 2019Article
- Dynamic imaging of cellular pH and redox homeostasis with a genetically encoded dual-functional biosensor, pHaROS, in yeast.The Journal of biological chemistry · 2019Article
- Functional Characterization of the Grapevine γ-Glutamyl Transferase/Transpeptidase (E.C. 2.3.2.2) Gene Family Reveals a Single Functional Gene Whose Encoded Protein Product Is Not Located in Either the Vacuole or Apoplast.Frontiers in plant science · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are derived from the most popular Aequorea victoria GFP (Av-GFP). Here, we present a novel fluorescent pH reporter protein from the orange seapen Ptilosarcus gurneyi (Pt-GFP) and compare its properties with pHluorins for expression and use in plants.
resultspHluorins have a higher pH-sensitivity. However, Pt-GFP has a broader pH-responsiveness, an excellent dynamic ratio range and a better acid stability. We demonstrate how Pt-GFP expressing Arabidopsis thaliana report cytosolic pH-clamp and changes of cytosolic pH in the response to anoxia and salt-stress.
conclusionPt-GFP appears to be the better choice when used for in vivo-recording of cellular pH in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.