ArticleGenes & development2006
Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 182 citations in OpenAlex.
- A Rab/Kinesin-12/kinase module couples vesicle delivery and phragmoplast dynamics during plant cell cytokinesis.The EMBO journal · 2026Article
- A MAPK cascade regulates nuclear migration during female gametogenesis to establish specific cell identity and rice female fertility.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cellular dynamics and molecular signaling networks of plant cytokinesis.Molecules and cells · 2026Review
- The N-terminal domain of COMPANION OF CELLULOSE SYNTHASE1 promotes microtubule array formation in Arabidopsis.Plant physiology · 2025Article
- Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone.Nature communications · 2024Article
- MAPK20-mediated ATG6 phosphorylation is critical for pollen development inHorticulture research · 2024Article
- Cortical microtubules contribute to division plane positioning during telophase in maize.The Plant cell · 2023Article
- Identification and Expression Analysis of MPK and MKK Gene Families in Pecan (International journal of molecular sciences · 2022Article
- Intracellular infection by symbiotic bacteria requires the mitotic kinase AURORA1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Functional analysis of mitogen-activated protein kinases (MAPKs) in potato under biotic and abiotic stress.Molecular breeding : new strategies in plant improvement · 2022Review
- MAP kinase cascades in plant development and immune signaling.EMBO reports · 2022Review
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis.Frontiers in plant science · 2022Article
- Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366.BMC genomics · 2021Article
- MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean.Science China. Life sciences · 2021Article
- The Protein Phosphatase PP2A Plays Multiple Roles in Plant Development by Regulation of Vesicle Traffic-Facts and Questions.International journal of molecular sciences · 2021Review
- Mapping proteome-wide targets of protein kinases in plant stress responses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Tomato locule number and fruit size controlled by natural alleles ofPlant direct · 2019Article
- The Solanum chacoense Fertilization-Related Kinase 3 (ScFRK3) is involved in male and female gametophyte development.BMC plant biology · 2019Article
- A dual role for cell plate-associated PI4Kβ in endocytosis and phragmoplast dynamics during plant somatic cytokinesis.The EMBO journal · 2019Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phragmoplast expansion were not known. Here, we show that NRK1/NTF6 phosphorylates the threonine residue at position 579 in NtMAP65-1a, a microtubule-associated (MT-associated) protein. Levels of phosphorylated NtMAP65-1 increase during late M phase of the cell cycle, when NRK1/NTF6 is activated. Phosphorylated NtMAP65-1 is concentrated at the equator of phragmoplast, as is NRK1/NTF6. Overexpression of mutant forms of NtMAP65-1a that cannot be phosphorylated by NRK1 delays progression of the M phase and phragmoplast expansion, also rendering phragmoplast structures resistant to an MT-depolymerizing drug. Phosphorylation of NtMAP65-1 by NRK1/NTF6 down-regulates its MT-bundling activity in vitro. These results suggest that phosphorylation of NtMAP65-1 by NRK1/NTF6 also reduces its MT-bundling activity in vivo, which enhances destabilization and turnover of MTs at the phragmoplast equator, perhaps facilitating phragmoplast expansion.
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