ArticleApplied and environmental microbiology2006
Quantitative real-time Legionella PCR for environmental water samples: data interpretation.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- PCR-BasedMicroorganisms · 2025Article
- Biosensing Technologies for DetectingMicroorganisms · 2024Review
- Virulence Genes and Biofilm Formation Among Legionella pneumophila Isolates Collected from Hospital Water Sources.Current microbiology · 2024Article
- Evaluation of a Natural Phytogenic Formulation as an Alternative to Pharmaceutical Zinc Oxide in the Diet of Weaned Piglets.Animals : an open access journal from MDPI · 2023Article
- Clinical and Laboratory Diagnosis of Legionella Pneumonia.Diagnostics (Basel, Switzerland) · 2023Review
- Design of an optical nanobiosensor for detection ofIranian journal of microbiology · 2022Article
- Evaluation of an Environmental Transport Medium forInternational journal of environmental research and public health · 2021Article
- Quantification of Legionella pneumophila by qPCR and culture in tap water with different concentrations of residual disinfectants and heterotrophic bacteria.The Science of the total environment · 2021Article
- Rapid Testing and Interventions to ControlMicroorganisms · 2021Article
- Article
- Diet supplementation with an organic acids-based formulation affects gut microbiota and expression of gut barrier genes in broilers.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2018Article
- Prevalence ofIranian journal of medical sciences · 2018Review
- On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.Water research · 2017Article
- Diagnostic testing for Legionnaires' disease.Annals of clinical microbiology and antimicrobials · 2017Review
- Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.BMC microbiology · 2017Article
- Evaluation of the microbiological quality of reclaimed water produced from a lagooning system.Environmental science and pollution research international · 2016Article
- Control of Legionella Contamination and Risk of Corrosion in Hospital Water Networks following Various Disinfection Procedures.Applied and environmental microbiology · 2016Article
- PCR method for the rapid detection and discrimination of Legionella spp. based on the amplification of pcs, pmtA, and 16S rRNA genes.Journal of applied genetics · 2016Article
- Evaluating exposure of pedestrians to airborne contaminants associated with non-potable water use for pavement cleaning.Environmental science and pollution research international · 2016Article
- Investigation of Legionella Contamination in Bath Water Samples by Culture, Amoebic Co-Culture, and Real-Time Quantitative PCR Methods.International journal of environmental research and public health · 2015Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative Legionella PCRs targeting the 16S rRNA gene (specific for the genus Legionella) and the mip gene (specific for the species Legionella pneumophila) were applied to a total of 223 hot water system samples (131 in one laboratory and 92 in another laboratory) and 37 cooling tower samples (all in the same laboratory). The PCR results were compared with those of conventional culture. 16S rRNA gene PCR results were nonquantifiable for 2.8% of cooling tower samples and up to 39.1% of hot water system samples, and this was highly predictive of Legionella CFU counts below 250/liter. PCR cutoff values for identifying hot water system samples containing >10(3) CFU/liter legionellae were determined separately in each laboratory. The cutoffs differed widely between the laboratories and had sensitivities from 87.7 to 92.9% and specificities from 77.3 to 96.5%. The best specificity was obtained with mip PCR. PCR cutoffs could not be determined for cooling tower samples, as the results were highly variable and often high for culture-negative samples. Thus, quantitative Legionella PCR appears to be applicable to samples from hot water systems, but the positivity cutoff has to be determined in each laboratory.
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