ArticleApplied and environmental microbiology2006
Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Proteomics as a complementary approach to measure norovirus infection in clinical samples.Virology · 2025Review
- An approach to zwitterionic peptide design for colorimetric detection of the Southampton norovirus SV3CP protease.The Analyst · 2023Article
- Deciphering the Virus Signal Within the Marine Dissolved Organic Matter Pool.Frontiers in microbiology · 2022Article
- Mass Spectrometry-Based System for Identifying and Typing Norovirus Major Capsid Protein VP1.Viruses · 2021Article
- Application and Perspectives of MALDI-TOF Mass Spectrometry in Clinical Microbiology Laboratories.Microorganisms · 2021Review
- A Survey of Analytical Techniques for Noroviruses.Foods (Basel, Switzerland) · 2020Review
- Introduction into nanotechnology and microbiology.Methods in microbiology · 2019Article
- New Microbiological Techniques in the Diagnosis of Bloodstream Infections.Deutsches Arzteblatt international · 2018Review
- Review
- Absolute quantification of norovirus capsid protein in food, water, and soil using synthetic peptides with electrospray and MALDI mass spectrometry.Journal of hazardous materials · 2015Article
- Serum peptidome variations in a healthy population: reference to identify cancer-specific peptides.PloS one · 2013Article
- [Mass spectrometry in the clinical microbiology laboratory].Enfermedades infecciosas y microbiologia clinica · 2012Review
- Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Capsid and Infectivity in Virus Detection.Food and environmental virology · 2009Article
- The measurement of charge for induction-based fluidic MALDI dispense event and nanoliter volume verification in real time.Journal of the American Society for Mass Spectrometry · 2009Article
- Autoimmune hypophysitis of SJL mice: clinical insights from a new animal model.Endocrinology · 2008Article
- Current literature in mass spectrometry.Journal of mass spectrometry : JMS · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mass spectrometry (MS) represents a rapid technique for the identification of microbial monocultures, and its adaptation to the detection of pathogens in real-world samples is a public health and homeland security priority. Norovirus, a leading cause of gastroenteritis in the world, is difficult to monitor because it cannot be cultured outside the human body. The detection of norovirus capsid protein was explored using three common MS-based methods: scanning of intact proteins, peptide mass fingerprinting, and peptide sequencing. Detection of intact target protein was limited by poor selectivity and sensitivity. Detection of up to 16 target peptides by peptide mass fingerprinting allowed for the reproducible and confident (P < 0.05) detection of the 56-kDa norovirus capsid protein in the range of 0.1 x 10(-12) to 50 x 10(-12) mol in authentic standards of recombinant norovirus virus-like particles (VLPs). To explore assay performance in complex matrixes, a non-gel-based, rapid method (2 to 3 h) for virus extraction from human stool was evaluated (72% +/- 12% recovery), and additional analyses were performed on norovirus-free stool extracts fortified with VLPs. Whereas peptide mass fingerprinting was rendered impractical by sample interferences, peptide sequencing using nanospray tandem MS facilitated unambiguous identification of > or =250 fmol of capsid protein in stool extracts. This is the first report on MS-based detection of norovirus, accomplished by using structurally identical, noninfective VLPs at clinically relevant concentrations. It represents an important milestone in the development of assays for surveillance of this category B bioterrorism agent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.