ArticleApplied and environmental microbiology2006
Assessment of the roles of LuxS, S-ribosyl homocysteine, and autoinducer 2 in cell attachment during biofilm formation by Listeria monocytogenes EGD-e.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 113 citations in OpenAlex.
- Antimicrobial and Pharmacokinetic Evaluation of Octanoic Acid Identified Through GC-MS Profiling of Lactiplantibacillus Plantarum 12 - 3 against LuxS-Mediated Quorum Sensing in Biofilms of Foodborne Pathogens Using Computational Approaches.Probiotics and antimicrobial proteins · 2026Article
- Daptomycin OvercomesInfection and drug resistance · 2026Article
- Exogenous L-Cysteine and Its Transport Through CtaP Play a Role in Biofilm Formation, Swimming Motility, and Swarming Motility ofFoods (Basel, Switzerland) · 2025Article
- Exploring the potential ofFrontiers in microbiology · 2025Article
- Bacterial aggregation facilitates internalin-mediated invasion ofFrontiers in cellular and infection microbiology · 2024Article
- Review
- Regulation of biofilm formation inFrontiers in bioengineering and biotechnology · 2023Article
- Andrographolide Inhibits Biofilm and Virulence inMolecules (Basel, Switzerland) · 2022Article
- Review
- Benzalkonium Chloride Adaptation Increases Expression of the Agr System, Biofilm Formation, and Virulence inFrontiers in microbiology · 2022Article
- Role ofInfection and immunity · 2020Article
- Links between S-adenosylmethionine and Agr-based quorum sensing for biofilm development in Listeria monocytogenes EGD-e.MicrobiologyOpen · 2020Article
- Antibodies generated against dextransucrase exhibit potential anticariostatic properties in Streptococcus mutans.Applied microbiology and biotechnology · 2020Article
- Efficacy of Synthetic Furanones onFoods (Basel, Switzerland) · 2019Article
- Global Transcriptional Response of Three Highly Acid-Tolerant Field Strains ofMicroorganisms · 2019Article
- Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance.Antimicrobial agents and chemotherapy · 2019Review
- Lactic Acid Bacteria (LAB) and Their Bacteriocins as Alternative Biotechnological Tools to Control Listeria monocytogenes Biofilms in Food Processing Facilities.Molecular biotechnology · 2018Review
- Highly Invasive Listeria monocytogenes Strains Have Growth and Invasion Advantages in Strain Competition.PloS one · 2015Article
- Intra- and inter-species interactions within biofilms of important foodborne bacterial pathogens.Frontiers in microbiology · 2015Review
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LuxS is responsible for the production of autoinducer 2 (AI-2), which is involved in the quorum-sensing response of Vibrio harveyi. AI-2 is found in several other gram-negative and gram-positive bacteria and is therefore considered a good candidate for an interspecies communication signal molecule. In order to determine if this system is functional in the gastrointestinal pathogen Listeria monocytogenes EGD-e, an AI-2 bioassay was performed with culture supernatants. The results indicated that this bacterium produces AI-2 like molecules. A potential ortholog of V. harveyi luxS, lmo1288, was found by performing sequence similarity searches and complementation experiments with Escherichia coli DH5alpha, a luxS null strain. lmo1288 was found to be a functional luxS ortholog involved in AI-2 synthesis. Indeed, interruption of lmo1288 resulted in loss of the AI-2 signal. Although no significant differences were observed between Lux1 and EGD-e with regard to planktonic growth (at 10 degrees C, 15 degrees C, 25 degrees C, and 42 degrees C), swimming motility, and phospholipase and hemolytic activity, biofilm culture experiments showed that under batch conditions between 25% and 58% more Lux1 cells than EGD-e cells were attached to the surface depending on the incubation time. During biofilm growth in continuous conditions after 48 h of culture, Lux1 biofilms were 17 times denser than EGD-e biofilms. Finally, our results showed that Lux1 accumulates more S-adenosyl homocysteine (SAH) and S-ribosyl homocysteine (SRH) in culture supernatant than the parental strain accumulates and that SRH, but not SAH or AI-2, is able to modify the number of attached cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.