ArticleApplied and environmental microbiology2006
Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers, 1 of them a synthesis that pooled it.
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Who cites it
122 citing papers in PubMed, 1 synthesis or guideline pooled it, 307 citations in OpenAlex.
- Prevalence ofFrontiers in microbiology · 2024Pooled it
- Effects of prey quality through the life cycle of the social amoeba Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Biofertilizer induces soil disease suppression by activating pathogen suppressive protist taxa.NPJ biofilms and microbiomes · 2026Article
- Isolation and Molecular Identification of Potentially Pathogenic Free-Living Amoeba in Tap Water Faucets in Quezon City, Philippines.Journal of parasitology research · 2026Article
- Adaptive evolution ofMicrobial genomics · 2026Article
- Geographic and Orientia infection status influence on the bacterial microbiome of free-living chiggers in North Carolina, USA.PloS one · 2026Article
- Soil-dwelling Naegleria enhances plant performance by stimulating beneficial bacterial functions in the rhizosphere.Nature communications · 2025Article
- Molecular Species Identification and Genotyping of Free-Living Amoebae in Soil of Recreational Mountain Areas in the Babiogórski National Park and Surroundings, Southern Poland.International journal of molecular sciences · 2025Article
- Genomic and structural insights into Jyvaskylavirus, the first giant virus isolated from Finland.eLife · 2025Article
- Acanthamoeba spp. in Dialysis Water: Assessing the Potential Risk of Transmission to Hemodialysis Patients.Acta parasitologica · 2025Article
- Protozoa-enhanced conjugation frequency alters the dissemination of soil antibiotic resistance.The ISME journal · 2025Article
- Diversity of Free-Living Amoebae in New Zealand Groundwater and Their Ability to Feed onPathogens (Basel, Switzerland) · 2024Article
- Screening of Antagonistic Bacillus against Brown Rot in Dendrocalamus latiflorus and Preparation of Applying Bacterial Suspension.The plant pathology journal · 2024Article
- Predatory protists reduce bacteria wilt disease incidence in tomato plants.Nature communications · 2024Article
- Homeostasis of cellular amino acids in Acanthamoeba castellanii exposed to different media under amoeba-bacteria coculture conditions.BMC microbiology · 2023Article
- Environmental stress promotes the persistence of facultative bacterial symbionts in amoebae.Ecology and evolution · 2023Article
- The Status of Molecular Analyses of Isolates ofMicroorganisms · 2023Review
- Interactions between free-living amoebae and Cryptosporidium parvum: an experimental study.Parasite (Paris, France) · 2023Article
- Premise plumbing bacterial communities in four European cities and their association withFrontiers in microbiomes · 2023Article
- Compromised Effectiveness of Thermal Inactivation ofMicroorganisms · 2022Article
62 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae (FLA) are ubiquitous organisms that have been isolated from various domestic water systems, such as cooling towers and hospital water networks. In addition to their own pathogenicity, FLA can also act as Trojan horses and be naturally infected with amoeba-resisting bacteria (ARB) that may be involved in human infections, such as pneumonia. We investigated the biodiversity of bacteria and their amoebal hosts in a hospital water network. Using amoebal enrichment on nonnutrient agar, we isolated 15 protist strains from 200 (7.5%) samples. One thermotolerant Hartmannella vermiformis isolate harbored both Legionella pneumophila and Bradyrhizobium japonicum. By using amoebal coculture with axenic Acanthamoeba castellanii as the cellular background, we recovered at least one ARB from 45.5% of the samples. Four new ARB isolates were recovered by culture, and one of these isolates was widely present in the water network. Alphaproteobacteria (such as Rhodoplanes, Methylobacterium, Bradyrhizobium, Afipia, and Bosea) were recovered from 30.5% of the samples, mycobacteria (Mycobacterium gordonae, Mycobacterium kansasii, and Mycobacterium xenopi) were recovered from 20.5% of the samples, and Gammaproteobacteria (Legionella) were recovered from 5.5% of the samples. No Chlamydia or Chlamydia-like organisms were recovered by amoebal coculture or detected by PCR. The observed strong association between the presence of amoebae and the presence of Legionella (P < 0.001) and mycobacteria (P = 0.009) further suggests that FLA are a reservoir for these ARB and underlines the importance of considering amoebae when water control measures are designed.
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