ArticleApplied and environmental microbiology2006
Assessment of the environmental fate of the biological control agent of fire blight, Pseudomonas fluorescens EPS62e, on apple by culture and real-time PCR methods.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Specific monitoring of viable plant growth-promoting Bacillus velezensis PH-023 in the rhizosphere of tomato.BMC microbiology · 2026Article
- Environmental conditions and ecological interactions among microorganisms isolated from diseased samples shape their biocontrol efficacy againstFrontiers in microbiology · 2026Article
- APlants (Basel, Switzerland) · 2025Article
- Monitoring the Persistence of Pseudomonas sivasensis Strain CF10PS3 in Cereal Fields.MicrobiologyOpen · 2024Article
- Colonization and population dynamics of total, viable, and culturable cells of two biological control strains applied to apricot, peach, and grapevine crops.Frontiers in microbiology · 2023Article
- Bacteria as Biological Control Agents of Plant Diseases.Microorganisms · 2022Review
- Molecular Assay Development to Monitor the Kinetics of Viable Populations of Two Biocontrol Agents,Biology · 2021Article
- Bee Vectoring: Development of the Japanese Orchard Bee as a Targeted Delivery System of Biological Control Agents for Fire Blight Management.Pathogens (Basel, Switzerland) · 2020Article
- Colonization of Beauveria bassiana 08F04 in root-zone soil and its biocontrol of cereal cyst nematode (Heterodera filipjevi).PloS one · 2020Article
- Exploring Rain as Source of Biological Control Agents for Fire Blight on Apple.Frontiers in microbiology · 2020Article
- Article
- Monitoring Viable Cells of the Biological Control Agent Lactobacillus plantarum PM411 in Aerial Plant Surfaces by Means of a Strain-Specific Viability Quantitative PCR Method.Applied and environmental microbiology · 2018Article
- Molecular Tools for MonitoringFrontiers in microbiology · 2018Review
- Improvement of fitness and efficacy of a fire blight biocontrol agent via nutritional enhancement combined with osmoadaptation.Applied and environmental microbiology · 2011Article
- Application of terminal restriction fragment length polymorphism (T-RFLP) analysis to monitor effect of biocontrol agents on rhizosphere microbial community of hot pepper (Capsicum annuum L.).Journal of microbiology (Seoul, Korea) · 2010Article
- Stress- and growth rate-related differences between plate count and real-time PCR data during growth of Listeria monocytogenes.Applied and environmental microbiology · 2009Article
- Prospects and limitations of microbial pesticides for control of bacterial and fungal pomefruit tree diseases.Trees (Berlin, Germany : West)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The colonization of apple blossoms and leaves by Pseudomonas fluorescens EPS62e was monitored in greenhouse and field trials using cultivable cell counting and real-time PCR. The real-time PCR provided a specific quantitative method for the detection of strain EPS62e. The detection level was around 10(2) cells g (fresh weight)(-1) and the standard curve was linear within a 5-log range. EPS62e actively colonized flowers reaching values from 10(7) to 10(8) cells per blossom. In apple flowers, no significant differences were observed between population levels obtained by real-time PCR and plating, suggesting that viable but nonculturable (VBNC) cells and residual nondegraded DNA were not present. In contrast, on apple leaves, where cultivable populations of EPS62e decreased with time, significant differences were observed between real-time PCR and plating. These differences indicate the presence of VBNC cells or nondegraded DNA after cell death. Therefore, the EPS62e population was under optimal conditions during the colonization of flowers but it was stressed and poorly survived on leaves. It was concluded that for monitoring this biological control agent, the combined use of cultivable cell count and real-time PCR is necessary.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.