Evidence map›Paper›PMID 16597882›Full record

ArticleJournal of clinical microbiology2006

Differentiation of Escherichia coli pathotypes by oligonucleotide spotted array.

Raghavan U M Palaniappan, Yu Zhang, David Chiu, Alfonso Torres, Chobi Debroy, Thomas S Whittam, Yung-Fu Chang

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 32 citations in OpenAlex.

  1. Antimicrobial Susceptibility Profiles ofAntibiotics (Basel, Switzerland) · 2025
    Article
  2. Article
  3. Review
  4. Nanomaterials to address the genesis of antibiotic resistance inFrontiers in cellular and infection microbiology · 2022
    Review
  5. Extraintestinal PathogenicPathogens (Basel, Switzerland) · 2021
    Review
  6. Article
  7. Review
  8. Invasion and translocation of uropathogenic Escherichia coli isolated from urosepsis and patients with community-acquired urinary tract infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Development of a microarray for identification of pathogenic Clostridium spp.Diagnostic microbiology and infectious disease · 2010
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Pathotyping Escherichia coli by using miniaturized DNA microarrays.Applied and environmental microbiology · 2007
    Article
  20. Microarrays in infection and immunity.Current opinion in chemical biology · 2007
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Raghavan U M PalaniappanDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Yu Zhang
David Chiu
Alfonso Torres
Chobi Debroy
Thomas S Whittam
Yung-Fu Chang
Cornell University · USMichigan State University · USNew York State College of Veterinary Medicine · USPennsylvania State University · US

Funding

FOOD &WATERBORNE DISEASES INTEGRATED RESEARCH NETWORK, ZOONOES RESEAR-N01AI030054 · NIAID · CORNELL UNIVERSITY · 2003 to 2003
–
NIAID NIH HHS N01 AI030054
6 · The paper itself

Abstract

To accurately determine the pathotypes of Escherichia coli strains, a comprehensive assessment of each strain that targets multiple genes is required. A new approach to the identification and characterization of E. coli pathotypes was developed by constructing gene-specific probes (70-mers) for not only the virulence genes associated with each E. coli pathotype but also the O157-, CFT073-, and K-12-specific and common genes of each pathotype. Analysis of oligonucleotide probes with reference and clinical isolates of E. coli pathotypes indicated that the array could differentiate the pathotypes on the basis of their virulence and specific gene patterns. Probes targeting common genes of E. coli were present in all the reference and clinical strains. Salmonella enterica subsp. enterica-specific genes and Salmonella core genes were used as negative controls. The entire E. coli pathotype showed reactivity to only 4 of the 81 Salmonella-specific gene probes. Characterization of the genetic and virulence profiles of a single strain by using probes for virulence factors and specific and common genes in the spotted array is an ideal diagnostic tool for determination of E. coli pathotypes and could also have a significant impact on the epidemiological analysis of E. coli infections.

Indexed as

Gene Expression ProfilingDNA, BacterialEscherichia coliEscherichia coli InfectionsOligonucleotide Array Sequence AnalysisOligonucleotide ProbesVirulenceDNA, BacterialOligonucleotide Probes

Identifiers

PMID16597882
PMCPMC1448672
OpenAlexW2100898850

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.