ArticleJournal of clinical microbiology2006
Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 120 citations in OpenAlex.
- Predominance of M1European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- ERS/ESCMID state-of-the-science statement on the role of rapid diagnostic tests for the diagnosis and management of community-acquired lower respiratory tract infections.The European respiratory journal · 2026Review
- Additional biomarkers and emm types associated with group A streptococcal toxic shock syndrome: a Japanese nationwide observational study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Observational
- Alterations in the prevalence and serotypes of Streptococcus pneumoniae in elderly patients with community-acquired pneumonia: a meta-analysis and systematic review.Pneumonia (Nathan Qld.) · 2025Review
- Microbiology of Acute Maxillary Sinusitis in Children.The Laryngoscope · 2021Article
- PCR coupled to electrospray ionization mass spectrometry for microbiological diagnosis and surveillance of ventilator-associated pneumonia.Experimental and therapeutic medicine · 2020Article
- Sequence Type Changes Associated with Decreasing Macrolide-Resistant Mycoplasma pneumoniae, Japan.Emerging infectious diseases · 2020Article
- Fully Automated Molecular Diagnostic System "Simprova" for Simultaneous Testing of Multiple Items.Scientific reports · 2020Article
- Article
- Detection of pathogens by real-time PCR in adult patients with acute exacerbation of bronchial asthma.BMC pulmonary medicine · 2017Article
- Simultaneous Detection of Nine Key Bacterial Respiratory Pathogens Using Luminex xTAGInternational journal of environmental research and public health · 2017Article
- DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan.BMC microbiology · 2016Article
- Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition.Journal of visualized experiments : JoVE · 2016Article
- Pathogens in COPD exacerbations identified by comprehensive real-time PCR plus older methods.International journal of chronic obstructive pulmonary disease · 2015Article
- Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia in children: clinical features and laboratory diagnosis.Italian journal of pediatrics · 2014Article
- Classification, identification, and clinical significance of Haemophilus and Aggregatibacter species with host specificity for humans.Clinical microbiology reviews · 2014Review
- Relationship between the GOLD combined COPD assessment staging system and bacterial isolation.International journal of chronic obstructive pulmonary disease · 2014Article
- Molecular beacons: a novel optical diagnostic tool.Archivum immunologiae et therapiae experimentalis · 2013Review
- Molecular detection of Mycoplasma pneumoniae by quantitative real-time PCR in patients with community acquired pneumonia.The Indian journal of medical research · 2013Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, real-time PCR with pathogen-specific molecular beacons (MB) and primers was evaluated for prediction of community-acquired pneumonia (CAP) causative agents, detecting six main CAP agents, Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Streptococcus pyogenes, simultaneously. The PCR assay was evaluated for fresh clinical specimens from infants and children (n = 389) and from adults (n = 40). The MB probes and primers are both pathogen specific, namely, the lytA gene for S. pneumoniae, the mip gene for L. pneumophila, and 16S rRNA genes for the remaining four organisms. DNA extraction of clinical specimens was performed with a commercially available EXTRAGEN II kit, and amplification was performed with Stratagene Mx3000P. The limit of detection for these pathogens ranged from 2 copies to 18 copies. The whole process from DNA extraction to the analysis was finished in less than 2 h. The obtained sensitivity and specificity of this real-time PCR study relative to those of conventional cultures were as follows: 96.2% and 93.2% for S. pneumoniae, 95.8% and 95.4% for H. influenzae, 100% and 100% for S. pyogenes, and 100% and 95.4% for M. pneumoniae, respectively. The sensitivity and specificity for M. pneumoniae relative to those of a serologic assay were 90.2% and 97.9%, respectively. In six clinical samples of C. pneumoniae, the real-time PCR gave positive predictable values, and in those cases, elevation of the titer value was also observed. In conclusion, we demonstrated that a real-time PCR assay with pathogen-specific MB is useful in identifying CAP causative agents rapidly and in examining the clinical course of empirical chemotherapy in a timely manner, supporting conventional culture methods.
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