ArticleJournal of clinical microbiology2006
Use of PCR and reverse line blot hybridization assay for rapid simultaneous detection and serovar identification of Chlamydia trachomatis.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Identification and characterization of mixed infections ofFrontiers in microbiology · 2022Article
- Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia.MicrobiologyOpen · 2018Article
- Article
- High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.PloS one · 2016Article
- Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women.Clinical microbiology reviews · 2015Review
- Application of HTB-SiHa cells transfected with a recombinant plasmid for external quality assessment of Chlamydia trachomatis PCR.Annals of laboratory medicine · 2014Article
- Chlamydia trachomatis genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of C. trachomatis genovar E and mixed infections.BMC infectious diseases · 2012Article
- Distribution of Chlamydia trachomatis serotypes in clinical urogenital samples from north-eastern Croatia.Current microbiology · 2012Article
- Multiplex PCR and reverse line blot hybridization assay (mPCR/RLB).Journal of visualized experiments : JoVE · 2011Article
- Anal infections with concomitant Chlamydia trachomatis genotypes among men who have sex with men in Amsterdam, the Netherlands.BMC infectious diseases · 2011Article
- Evaluation of a novel Chlamydia trachomatis microsphere suspension assay for detection and genotyping of the different serovars in clinical samples.The Journal of molecular diagnostics : JMD · 2011Article
- Development and evaluation of an ompA quantitative real-time PCR assay for Chlamydia trachomatis serovar determination.Journal of clinical microbiology · 2010Article
- Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay.Journal of clinical microbiology · 2009Article
- Rapid detection of Chlamydia trachomatis and typing of the Lymphogranuloma venereum associated L-Serovars by TaqMan PCR.BMC infectious diseases · 2008Article
- Genotyping of Chlamydia trachomatis by microsphere suspension array.Journal of clinical microbiology · 2008Article
- Evaluation of a novel PCR-based assay for detection and identification of Chlamydia trachomatis serovars in cervical specimens.Journal of clinical microbiology · 2007Article
- A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.The Journal of molecular diagnostics : JMD · 2007Article
- Development of real-time PCR assays for genotyping of Chlamydia trachomatis.Journal of clinical microbiology · 2007Article
- Lateral gene transfer in vitro in the intracellular pathogen Chlamydia trachomatis.Journal of bacteriology · 2007Article
- Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to develop and evaluate multiplex and nested PCR-reverse line blot (RLB) hybridization assays for detection and serovar identification of Chlamydia trachomatis. Two sets of primers targeting the VD2 region of the omp1 gene and one set targeting the cryptic plasmid were designed for use in multiplex (both targets) and nested PCR (omp1 only). For the RLB assay, labeled omp1 amplicons were hybridized to a membrane containing probes specific for 15 C. trachomatis serovars. The assays were used to test 429 clinical specimens, which had been previously tested for C. trachomatis using the COBAS AMPLICOR system. Specimens were tested without knowledge of the COBAS AMPLICOR result. Of 205 specimens that were positive by COBAS AMPLICOR, 201 (98%) were positive by multiplex PCR-RLB and 188 (92%) were also positive by omp1 nested PCR-RLB. In addition, three of 224 COBAS AMPLICOR-negative specimens were positive by omp1 nested PCR-RLB. One hundred sixty-six of 191 (87%) specimens in which C. trachomatis serovars were identified contained only one serovar and 25 (13%) contained two or three serovars. Serovars D, E, and F were found in 31 (16%), 83 (43%), and 51 (27%) specimens, respectively. Serovar E (41%) was the most commonly identified single serovar. Serovars J and K were found alone uncommonly (<2% each), but 18 of 25 (72%) specimens with multiple C. trachomatis serovars contained one or both (10 specimens) of these serovars. The nested (ompI) PCR-RLB is a specific and sensitive method for simultaneous detection and serovar identification of C. trachomatis, which can reliably identify mixed C. trachomatis serovars. It is suitable for use in epidemiological studies.
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