ArticleJournal of clinical microbiology2006
Use of TaqMan real-time reverse transcription-PCR for rapid detection, quantification, and typing of norovirus.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 249 citations in OpenAlex.
- Seasonal dynamics and genetic diversity of non-polio enteroviruses in hospitalized children with acute gastroenteritis in Pune, India.Scientific reports · 2026Article
- Identifying intra-hospital Norovirus GII transmission using whole-genome sequencing.Genome medicine · 2026Article
- Norovirus infection associated with unusual fatal case of Guillain-Barré syndrome in the community outbreak in Pune, India.Virology journal · 2025Article
- Detection of human noroviruses in sewage by next generation sequencing in Shandong Province, 2019-2021.Virology journal · 2025Article
- Machine Learning and Imputation to Characterize Human Norovirus Genotype Susceptibility to Sodium Hypochlorite.Food and environmental virology · 2024Article
- Article
- Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity.Viruses · 2022Article
- Norovirus GII.3[P12] Outbreak Associated with the Drinking Water Supply in a Rural Area in Galicia, Spain, 2021.Microbiology spectrum · 2022Article
- Secretor Status Strongly Influences the Incidence of Symptomatic Norovirus Infection in a Genotype-Dependent Manner in a Nicaraguan Birth Cohort.The Journal of infectious diseases · 2022Article
- Norovirus transmission mitigation strategies during simulated produce harvest and packing.International journal of food microbiology · 2021Article
- Norovirus GII.2[P16] strain in Shenzhen, China: a retrospective study.BMC infectious diseases · 2021Article
- Article
- Virucidal Efficacy of Olanexidine Gluconate as a Hand Antiseptic Against Human Norovirus.Food and environmental virology · 2020Article
- Applicability of crAssphage, pepper mild mottle virus, and tobacco mosaic virus as indicators of reduction of enteric viruses during wastewater treatment.Scientific reports · 2020Article
- Impact of long-term storage of clinical samples collected from 1996 to 2017 on RT-PCR detection of norovirus.Journal of virological methods · 2019Article
- Epidemiological, Molecular, and Clinical Features of Norovirus Infections among Pediatric Patients in Qatar.Viruses · 2019Article
- No evidence of an increase in the incidence of norovirus gastroenteritis hospitalizations in young children after the introduction of universal rotavirus immunization in Israel.Human vaccines & immunotherapeutics · 2019Article
- A swimming pool-associated outbreak of pharyngoconjunctival fever caused by human adenovirus type 4 in Beijing, China.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2018Article
- Surveillance of Noroviruses in Rio De Janeiro, Brazil: Occurrence of New GIV Genotype in Clinical and Wastewater Samples.Food and environmental virology · 2018Article
- Syndromic Panel-Based Testing in Clinical Microbiology.Clinical microbiology reviews · 2018Review
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noroviruses (NoVs) are the most commonly identified cause of outbreaks and sporadic cases of acute gastroenteritis. We evaluated and optimized NoV-specific TaqMan real-time reverse transcription (RT)-PCR assays for the rapid detection and typing of NoV strains belonging to genogroups GI and GII and adapted them to the LightCycler platform. We expanded the detection ability of the assays by developing an assay that detects the GIV NoV strain. The assays were validated with 92 clinical samples and 33 water samples from confirmed NoV outbreaks and suspected NoV contamination cases. The assays detected NoV RNA in all of the clinical specimens previously confirmed positive by conventional RT-PCR and sequencing. Additionally, the TaqMan assays successfully detected NoV RNA in water samples containing low viral concentrations and inhibitors of RT and/or PCR, whereas the conventional method with region B primers required dilution of the inhibitors. By means of serially diluted NoV T7 RNA transcripts, a potential detection limit of <10 transcript copies per reaction mixture was observed with the GII assay and a potential detection limit of <100 transcript copies per reaction mixture was observed with the GI assay. These results and the ability to detect virus in water that was negative by RT-PCR demonstrate the higher sensitivity of the TaqMan assay compared with that of a conventional RT-PCR assay. The TaqMan methods dramatically decrease the turnaround time by eliminating post-PCR processing. These assays have proven useful in assisting scientists in public health and diagnostic laboratories report findings quickly to outbreak management teams.
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