ArticleJournal of clinical microbiology2006
Follow-up on diagnostic proficiency of laboratories equipped to perform orthopoxvirus detection and quantification by PCR: the second international external quality assurance study.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 55 citations in OpenAlex.
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- Sonography of monkeypox: comment.Journal of ultrasound · 2024Article
- Comments on the article "Monkey pox and female sexual health".Revista colombiana de obstetricia y ginecologia · 2023Article
- Mpox vaccination for men who have sex with men.Human vaccines & immunotherapeutics · 2023Article
- Proctitis in patients with monkeypox infection.Techniques in coloproctology · 2023Article
- Comment on article on monkeypox, covid and diabetes.Primary care diabetes · 2023Article
- Epidemiological and clinical characteristics of mpox cases: correspondence.Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil · 2023Article
- Laboratory Diagnosis for Monkeypox: Clinical Practice and Important Points to Be Recognized.Advances in experimental medicine and biology · 2023Article
- The European network of Biosafety-Level-4 laboratories: enhancing European preparedness for new health threats.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2009Review
- Coordinated implementation of chikungunya virus reverse transcription-PCR.Emerging infectious diseases · 2009Article
- Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.The Journal of infectious diseases · 2007Article
- Use of internally controlled real-time genome amplification for detection of variola virus and other orthopoxviruses infecting humans.Journal of clinical microbiology · 2006Article
- Monkeypox Outbreaks in Non-Endemic Countries: Correspondence.Avicenna journal of medical biotechnologyArticle
- Ocular manifestations of monkeypox: correspondence.Arquivos brasileiros de oftalmologiaArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two years after the first external quality assurance study on bioterrorism-relevant viruses, we have conducted a follow-up study on orthopoxvirus detection by PCR. Thirty-three laboratories (27 European, 4 Austral-Asian, and 2 American) participated. Samples contained 0 to 40,000,000 DNA copies of lyophilized monkeypox, cowpox, and vaccinia virus per ml. Laboratories achieved a >80% detection chance above 56,234 copies per ml. Global sensitivity was not significantly improved over that of the first study. Twenty-seven and 9 participants, respectively, were able to genotype and quantify virus. Four of 27 genotyping results were incorrect. Quantification accuracy was significantly better for vaccinia virus than for the other viruses. False-positive results occurred in 22 (11.8%) of all 186 tests on negative samples, but 18 of these were contributed by only five laboratories. Fifty-five percent of laboratories could appropriately detect PCR inhibition. The use of either real-time PCR or commercial diagnostic kits had significant positive influence on laboratory performance.
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