ArticleNucleic acids research2006
Molecular beacons with intrinsically fluorescent nucleotides.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures.Chembiochem : a European journal of chemical biology · 2021Article
- Fluorescent base analogues in gapmers enable stealth labeling of antisense oligonucleotide therapeutics.Scientific reports · 2021Article
- Fluorescent nucleobases as tools for studying DNA and RNA.Nature chemistry · 2017Review
- Enzymatic incorporation and utilization of an emissive 6-azauridine.Organic & biomolecular chemistry · 2017Article
- Photophysical properties of pyrrolocytosine, a cytosine fluorescent base analogue.Physical chemistry chemical physics : PCCP · 2016Article
- Tryptophan-based fluorophores for studying protein conformational changes.Bioorganic & medicinal chemistry · 2014Article
- Fluorescent biphenyl derivatives of phenylalanine suitable for protein modification.Biochemistry · 2013Article
- Detection of dihydrofolate reductase conformational change by FRET using two fluorescent amino acids.Journal of the American Chemical Society · 2013Article
- G-quadruplexes for luminescent sensing and logic gates.Nucleic acids research · 2013Review
- Quadruplex formation as a molecular switch to turn on intrinsically fluorescent nucleotide analogs.Nucleic acids research · 2013Article
- Fluorescent hybridization probes for nucleic acid detection.Analytical and bioanalytical chemistry · 2012Review
- Synthetic nucleotides as probes of DNA polymerase specificity.Journal of nucleic acids · 2012Article
- Three-dimensional models of the oligomeric human asialoglycoprotein receptor (ASGP-R).International journal of molecular sciences · 2010Article
- Fluorescent analogs of biomolecular building blocks: design, properties, and applications.Chemical reviews · 2010Review
- FRET enabled real time detection of RNA-small molecule binding.Journal of the American Chemical Society · 2009Article
- An emissive C analog distinguishes between G, 8-oxoG, and T.Organic letters · 2009Article
- DNA hairpins containing the cytidine analog pyrrolo-dC: structural, thermodynamic, and spectroscopic studies.Biophysical journal · 2009Article
- Pyrene excimer signaling molecular beacons for probing nucleic acids.Journal of the American Chemical Society · 2008Article
- Article
- Using force spectroscopy analysis to improve the properties of the hairpin probe.Nucleic acids research · 2007Article
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Authors and funding
5 authors.
Funding
Abstract
We report the design, synthesis and characterization of a novel molecular beacon (MB-FB) which uses the fluorescent bases (FB) 2-aminopurine (AP) and pyrrolo-dC (P-dC) as fluorophores. Because the quantum yield of these FB depend on hybridization with complementary target, the fluorescent properties of MB-FB were tuned by placing the FB site specifically within the MB such that hybridization with complementary sequence switches from single strand to double strand for AP and vice versa for P-dC. The MB-FB produces a ratiometric fluorescence increase (the fluorescence emission of P-dC over that of AP in the presence and absence of complementary sequence) of 8.5 when excited at 310 nm, the maximum absorption of AP. This ratiometric fluorescence is increased to 14 by further optimizing excitation (325 nm). The fluorescence lifetime is also affected by the addition of target, producing a change in the long-lived component from 6.5 to 8.7 ns (Exc. 310 nm, Em. 450 nm). Thermal denaturation profiles monitored at 450 nm (P-dC emission) show a cooperative denaturation of the MB-FB with a melting temperature of 53 degrees C. The thermal denaturation profile of MB-FB hybridized with its target shows a marked fluorescence reduction at 53 degrees C, consistent with a transition from double stranded helix to random coil DNA.
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