ArticleThe Journal of cell biology1991
Transcription of the muscle regulatory gene Myf4 is regulated by serum components, peptide growth factors and signaling pathways involving G proteins.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 82 citations in OpenAlex.
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- cAMP signaling in skeletal muscle adaptation: hypertrophy, metabolism, and regeneration.American journal of physiology. Endocrinology and metabolism · 2012Review
- The myogenic kinome: protein kinases critical to mammalian skeletal myogenesis.Skeletal muscle · 2011Article
- Dual roles of smad proteins in the conversion from myoblasts to osteoblastic cells by bone morphogenetic proteins.The Journal of biological chemistry · 2010Article
- Glucocorticoid inhibition of C2C12 proliferation rate and differentiation capacity in relation to mRNA levels of the MRF gene family.Molecular biology reports · 2000Article
- Involvement of type 4 cAMP-phosphodiesterase in the myogenic differentiation of L6 cells.Molecular biology of the cell · 1999Article
- CDF-1-mediated repression of cell cycle genes targets a specific subset of transactivators.Nucleic acids research · 1997Article
- Genetic variation in the porcine myogenin gene locus.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Expression of the Gs protein alpha-subunit disrupts the normal program of differentiation in cultured murine myogenic cells.The Journal of clinical investigation · 1997Article
- Influence of PDGF-BB on proliferation and transition through the MyoD-myogenin-MEF2A expression program during myogenesis in mouse C2 myoblasts.Growth factors (Chur, Switzerland) · 1997Article
- A novel E1A domain mediates skeletal-muscle-specific enhancer repression independently of pRB and p300 binding.Molecular and cellular biology · 1996Article
- The kinase inhibitor iso-H7 stimulates rat satellite cell differentiation through a non-protein kinase C pathway by increasing myogenin expression level.Cell biology and toxicology · 1996Article
- Lysophosphatidic acid and bFGF control different modes in proliferating myoblasts.The Journal of cell biology · 1996Article
- Thyroid hormone inhibits fatty acid synthase gene transcription in chicken liver.Molecular and cellular biochemistry · 1995Article
- Expression of AMD 1, a gene for a MyoD 1-related factor in the ascidian Halocynthia roretzi.Roux's archives of developmental biology : the official organ of the EDBO · 1994Article
- Upstream sequences of the myogenin gene convey responsiveness to skeletal muscle denervation in transgenic mice.Nucleic acids research · 1993Article
- Mapping of myogenin transcription during embryogenesis using transgenes linked to the myogenin control region.The Journal of cell biology · 1992Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The muscle regulatory protein myogenin accumulates in differentiating muscle cells when the culture medium is depleted for serum. To investigate the regulation of myogenin gene expression, we have isolated and characterized the Myf4 gene which encodes the human homologue of murine myogenin. Serum components, basic FGF (b-FGF), transforming growth factor beta (TGF-beta), and EGF, agents which suppress differentiation of muscle cells in vitro, down-regulate the activity of the Myf4 gene, suggesting that it constitutes a nuclear target for the negative control exerted by these factors. The 5' upstream region containing the Myf4 promoter confers activity to a CAT reporter plasmid in C2C12 myotubes but not in fibroblasts and undifferentiated myoblasts. Unidirectional 5' deletions of the promoter sequence reveal that integral of 200 nucleotides upstream of the transcriptional start site are sufficient for cell type-specific expression. The forced expression of the muscle determining factors, MyoD1, Myf5, and Myf6 and to a lesser degree Myf4, results in the transactivation of the Myf4 promoter in C3H mouse 10T1/2 fibroblasts. Pathways potentially involved in conveying signals from the cell-surface receptors to the Myf4 gene were probed with pertussis- and cholera toxin, forskolin, and cAMP. Dibutyryl-cAMP and compounds that stimulate adenylate cyclase inhibit the endogenous Myf4 gene and the Myf4 promoter in CAT and LacZ reporter constructs. Conversely, pertussis toxin which modifies Gi protein stimulates Myf4 gene expression. In summary, our data provide evidence that the muscle-specific expression of the Myf4 gene is subject to negative control by serum components, growth factors and a cAMP-dependent intracellular mechanism. Positive control is exerted by a pertussis toxin-sensitive pathway that presumably involves G proteins.
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