ArticleProceedings of the National Academy of Sciences of the United States of America1986
Visualization of the Ca-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos.The Journal of cell biology · 1996Article
- Ca2+ triggers premature inactivation of the cdc2 protein kinase in permeabilized sea urchin embryos.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- A 62-kD protein required for mitotic progression is associated with the mitotic apparatus during M-phase and with the nucleus during interphase.The Journal of cell biology · 1992Article
- Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.The Journal of cell biology · 1991Article
- Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.The Journal of cell biology · 1991Article
- A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.The Journal of cell biology · 1989Article
- The distribution, abundance and subcellular localization of kinesin.The Journal of cell biology · 1989Article
- Calcium regulation of pigment transport in vitro.The Journal of cell biology · 1988Article
- Centrosome detection in sea urchin eggs with a monoclonal antibody against Drosophila intermediate filament proteins: characterization of stages of the division cycle of centrosomes.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoclonal antibodies have been obtained to components of Ca(2+)-sequestering vesicles from the endoplasmic reticulum of HeLa cells by isolating hybridomas that were generated by the in vitro immunization of lymphocytes followed by fusion with plasmocytoma cells. One of these monoclonal antibodies specifically labels punctate structures which appear in the mitotic apparatus of sea urchin eggs at the beginning of prophase and disappear upon the completion of cytokinesis. The antibody inhibits the Ca(2+) uptake of the membrane system in vitro. It reacts with one 46-kDa protein out of the complex protein mixture from the membrane fraction. We take all this as evidence that in fact a specific Ca(2+)-transport system is part of the mitotic apparatus, that such a system is very conserved, and that it is most probably derived from the endoplasmic reticulum.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.