ArticleProceedings of the National Academy of Sciences of the United States of America1985
Fluctuation of the Ca-sequestering activity of permeabilized sea urchin embryos during the cell cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 33 citations in OpenAlex.
- Intracellular and extracellular pH and Ca are bound to control mitosis in the early sea urchin embryo via ERK and MPF activities.PloS one · 2013Article
- Ca2+ triggers premature inactivation of the cdc2 protein kinase in permeabilized sea urchin embryos.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts.Biophysical journal · 1994Article
- Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Regulation of glucose-6-phosphate dehydrogenase activity in sea urchin eggs by reversible association with cell structural elements.The Journal of cell biology · 1986Article
- Visualization of the Ca-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.The Journal of cell biology · 1985Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have followed the sequestration of Ca(2+) by intracellular compartments in sea urchin embryos through the first cell cycles. To gain biochemical access to these compartments, the embryos were permeabilized by brief exposure to an intense electric field. Sequestration was determined as the retention of tracer, (45)Ca, after filtration of aliquots on Millipore filters. The permeabilized cells sequester Ca(2+) at a constant rate for at least 20 min, with the following characteristics: (i) ATP is required. (ii) Sequestration occurs at Ca(2+) levels corresponding to those estimated in vivo. (iii) The Ca(2+) concentration dependence of sequestration and its insensitivity to mitochondrial poisons imply that the activity derives from a single, nonmitochondrial transport system. The Ca(2+)-sequestering activities of embryos that are permeabiized at successive stages of the first cell cycle (one-cell stage) progressively increase to 5 times the initial level. The rate of sequestration is maximal during telophase and, in some populations of zygotes, is nearly as great throughout prophase. Over the course of the second cell cycle (two-cell stage), the activity undergoes a 2-fold oscillation that bears the same temporal relationship to mitosis as the previous fluctuation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.