ArticleJournal of bacteriology2006
Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- A metabolite morphogen coordinates multicellular development inScience advances · 2026Article
- Cell differentiation, aging, and death in spatially organized yeast communities: mechanisms and consequences.Cell death and differentiation · 2025Review
- Harnessing emergent properties of microbial consortia for Agriculture: Assembly of the Xilonen SynCom.Biofilm · 2025Article
- Pectic homogalacturonan sensed byiScience · 2023Article
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- The roles of intracellular and extracellular calcium iniScience · 2022Article
- The environment topography alters the way to multicellularity inScience advances · 2021Article
- Bacillus subtilis EA-CB0575 genome reveals clues for plant growth promotion and potential for sustainable agriculture.Functional & integrative genomics · 2020Article
- Environment Shapes the Intra-species Diversity of Bacillus subtilis Isolates.Microbial ecology · 2020Article
- Stress-Induced, Highly Efficient, Donor Cell-Dependent Cell-to-Cell Natural Transformation in Bacillus subtilis.Journal of bacteriology · 2018Article
- Effects of growth rate and promoter activity on single-cell protein expression.Scientific reports · 2017Article
- Termination factor Rho: From the control of pervasive transcription to cell fate determination in Bacillus subtilis.PLoS genetics · 2017Article
- Heterologous expression of antigenic peptides in Bacillus subtilis biofilms.Microbial cell factories · 2016Article
- Regulatory mechanisms link phenotypic plasticity to evolvability.Scientific reports · 2016Article
- Spatio-Temporal Evolution of Sporulation in Bacillus thuringiensis Biofilm.Frontiers in microbiology · 2016Article
- A fluorescence-based bioassay for antibacterials and its application in screening natural product extracts.The Journal of antibiotics · 2015Article
- Impact of domestication in the production of the class II lanthipeptide lichenicidin by Bacillus licheniformis I89.Current microbiology · 2015Article
- On growth and form of Bacillus subtilis biofilms.Interface focus · 2014Article
- Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.Molecular microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spore-forming bacterium Bacillus subtilis is able to form highly organized multicellular communities called biofilms. This coordinated bacterial behavior is often lost in domesticated or laboratory strains as a result of planktonic growth in rich media for many generations. However, we show here that the laboratory strain B. subtilis 168 is still capable of forming spatially organized multicellular communities on minimal medium agar plates, exemplified by colonies with vein-like structures formed by elevated bundles of cells. In line with the current model for biofilm formation, we demonstrate that overproduction of the phosphorelay components KinA and Spo0A stimulates bundle formation, while overproduction of the transition state regulators AbrB and SinR leads to repression of formation of elevated bundles. Time-lapse fluorescence microscopy studies of B. subtilis green fluorescent protein reporter strains show that bundles are preferential sites for spore formation and that flat structures surrounding the bundles contain vegetative cells. The elevated bundle structures are formed prior to sporulation, in agreement with a genetic developmental program in which these processes are sequentially activated. Perturbations of the phosphorelay by disruption and overexpression of genes that lead to an increased tendency to sporulate result in the segregation of sporulation mutations and decreased heat resistance of spores in biofilms. These results stress the importance of a balanced control of the phosphorelay for biofilm and spore development.
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