ArticleJournal of bacteriology2006
Upregulated transcription of plasmid and chromosomal ribulose monophosphate pathway genes is critical for methanol assimilation rate and methanol tolerance in the methylotrophic bacterium Bacillus methanolicus.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 103 citations in OpenAlex.
- Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces COMicrobial biotechnology · 2026Article
- Repair of DNA and protein damages caused by formaldehyde improves methanol assimilation.Fundamental research · 2026Article
- Development of a markerless tool for targeted chromosome modification in the thermophilic and methylotrophic bacterium Bacillus methanolicus.Microbial cell factories · 2025Article
- Identification of MscS as a Key L-Glutamate Exporter in Bacillus methanolicus.Microbial biotechnology · 2025Article
- The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CONature communications · 2023Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol.Microbial biotechnology · 2023Article
- Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.Microorganisms · 2022Review
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains.Frontiers in bioengineering and biotechnology · 2022Review
- Identification and Cross-Characterisation of Artificial Promoters and 5' Untranslated Regions inFrontiers in bioengineering and biotechnology · 2022Article
- Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading StrainInternational journal of molecular sciences · 2021Article
- Developing a Riboswitch-Mediated Regulatory System for Metabolic Flux Control in ThermophilicInternational journal of molecular sciences · 2021Article
- Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by ThermophilicFrontiers in bioengineering and biotechnology · 2021Article
- Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases ofFrontiers in microbiology · 2021Article
- Genomic and Transcriptomic Investigation of the Physiological Response of the MethylotrophFrontiers in microbiology · 2021Article
- Improving the Methanol Tolerance of anFrontiers in microbiology · 2021Article
- Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus.mSystems · 2020Article
- Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus.Microbial cell factories · 2020Article
- Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum.Communications biology · 2020Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The natural plasmid pBM19 carries the key mdh gene needed for the oxidation of methanol into formaldehyde by Bacillus methanolicus. Five more genes, glpX, fba, tkt, pfk, and rpe, with deduced roles in the cell primary metabolism, are also located on this plasmid. By using real-time PCR, we show that they are transcriptionally upregulated (6- to 40-fold) in cells utilizing methanol; a similar induction was shown for two chromosomal genes, hps and phi. These seven genes are involved in the fructose bisphosphate aldolase/sedoheptulose bisphosphatase variant of the ribulose monophosphate (RuMP) pathway for formaldehyde assimilation. Curing of pBM19 causes higher methanol tolerance and reduced formaldehyde tolerance, and the methanol tolerance is reversed to wild-type levels by reintroducing mdh. Thus, the RuMP pathway is needed to detoxify the formaldehyde produced by the methanol dehydrogenase-mediated conversion of methanol, and the in vivo transcription levels of mdh and the RuMP pathway genes reflect the methanol tolerance level of the cells. The transcriptional inducer of hps and phi genes is formaldehyde, and not methanol, and introduction of multiple copies of these two genes into B. methanolicus made the cells more tolerant of growth on high methanol concentrations. The recombinant strain also had a significantly higher specific growth rate on methanol than the wild type. While pBM19 is critical for growth on methanol and important for formaldehyde detoxification, the maintenance of this plasmid represents a burden for B. methanolicus when growing on mannitol. Our data contribute to a new and fundamental understanding of the regulation of B. methanolicus methylotrophy.
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