ArticleJournal of bacteriology2006
Detailed genomic analysis of the Wbeta and gamma phages infecting Bacillus anthracis: implications for evolution of environmental fitness and antibiotic resistance.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 106 citations in OpenAlex.
- Identification and characterization of two Bacillus anthracis bacteriophages.Archives of virology · 2024Article
- Genomic and taxonomic evaluation of 38 Treponema prophage sequences.BMC genomics · 2024Article
- Thinking Phage Innovations Through Evolution and Ecology.PHAGE (New Rochelle, N.Y.) · 2024Review
- Metagenomic Sequencing Identified Specific Bacteriophage Signature Discriminating between Healthy and Diarrheal Neonatal Piglets.Nutrients · 2023Article
- Phage Adsorption to Gram-Positive Bacteria.Viruses · 2023Review
- Phage-Encoded Sigma Factors Alter Bacterial Dormancy.mSphere · 2022Article
- Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation byPathogens (Basel, Switzerland) · 2022Article
- Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.Science advances · 2022Article
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- In Silico Characterisation of Putative Prophages inMicroorganisms · 2022Article
- Presence and Persistence of Putative Lytic and Temperate Bacteriophages in Vaginal Metagenomes from South African Adolescents.Viruses · 2021Article
- The roles of antimicrobial resistance, phage diversity, isolation source and selection in shaping the genomic architecture ofMicrobial genomics · 2021Article
- A catalog of tens of thousands of viruses from human metagenomes reveals hidden associations with chronic diseases.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Causes and Consequences of Bacteriophage Diversification via Genetic Exchanges across Lifestyles and Bacterial Taxa.Molecular biology and evolution · 2021Article
- Engineered Bacteriophage Therapeutics: Rationale, Challenges and Future.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Article
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- Rapid Microscopic Detection ofMicroorganisms · 2020Article
- Phage-Mediated Molecular Detection (PMMD): A Novel Rapid Method for Phage-Specific Bacterial Detection.Viruses · 2020Article
- New GenusViruses · 2019Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Phage-mediated lysis has been an essential laboratory tool for rapidly identifying Bacillus anthracis for more than 40 years, relying on the gamma phage derivative of a Bacillus cereus prophage called W. The complete genomic sequences of the temperate W phage, referred to as Wbeta, and its lytic variant gamma were determined and found to encode 53 open reading frames each, spanning 40,864 bp and 37,373 bp, respectively. Direct comparison of the genomes showed that gamma evolved through mutations at key loci controlling host recognition, lysogenic growth, and possibly host phenotypic modification. Included are a cluster of point mutations at the gp14 tail fiber locus of gamma, encoding a protein that, when fused to green fluorescent protein, binds specifically to B. anthracis. A large 2,003-bp deletion was also identified at the gamma lysogeny module, explaining its shift from a temperate to a lytic lifestyle. Finally, evidence of recombination was observed at a dicistronic Wbeta locus, encoding putative bacterial cell surface-modifying proteins, replaced in gamma with a locus, likely obtained from a B. anthracis prophage, encoding demonstrable fosfomycin resistance. Reverse transcriptase PCR analysis confirmed strong induction at the dicistronic Wbeta locus and at four other phage loci in B. anthracis and/or B. cereus lysogens. In all, this study represents the first genomic and functional description of two historically important phages and is part of a broader investigation into contributions of phage to the B. anthracis life cycle. Initial findings suggest that lysogeny of B. anthracis promotes ecological adaptation, rather than virulence, as with other gram-positive pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.