ArticleThe Journal of cell biology2006
The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers, 1 of them a synthesis that pooled it.
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Who cites it
131 citing papers in PubMed, 1 synthesis or guideline pooled it, 209 citations in OpenAlex.
- Meta-analysis reveals conserved cell cycle transcriptional network across multiple human cell types.BMC genomics · 2017Pooled it
- Contribution of dominant and recessive model effects to the genetic architecture of Idiopathic Pulmonary Fibrosis.medRxiv : the preprint server for health sciences · 2026Article
- KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.Cell reports · 2025Article
- Dysregulated miRNAs Targeting Adiponectin Signaling in Colorectal Cancer.International journal of molecular sciences · 2025Review
- Identification and Biological Evaluation of a Novel CLK4 Inhibitor Targeting Alternative Splicing in Pancreatic Cancer Using Structure-Based Virtual Screening.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A Comprehensive Analysis of the Role of DSN1 in Pan-Cancer Prognosis and Immunotherapy.Journal of Cancer · 2025Article
- CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.Life science alliance · 2025Article
- DSN1 drives breast cancer progression via cell cycle regulation: diagnostic and therapeutic implications.Frontiers in oncology · 2025Article
- Review
- Centromeres in cancer: Unraveling the link between chromosomal instability and tumorigenesis.Medical oncology (Northwood, London, England) · 2024Review
- A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development.Current biology : CB · 2024Article
- DNAJC9 prevents CENP-A mislocalization and chromosomal instability by maintaining the fidelity of histone supply chains.The EMBO journal · 2024Article
- A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development.bioRxiv : the preprint server for biology · 2024Article
- A multidimensional analysis of ZW10 interacting kinetochore protein in human tumors.American journal of cancer research · 2024Article
- Netrin 1 directs vascular patterning and maturity in the developing kidney.Development (Cambridge, England) · 2023Article
- Review
- Mobility of kinetochore proteins measured by FRAP analysis in living cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2022Article
- Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions.Nature communications · 2022Article
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- Review
71 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
During cell division, kinetochores form the primary chromosomal attachment sites for spindle microtubules. We previously identified a network of 10 interacting kinetochore proteins conserved between Caenorhabditis elegans and humans. In this study, we investigate three proteins in the human network (hDsn1Q9H410, hNnf1PMF1, and hNsl1DC31). Using coexpression in bacteria and fractionation of mitotic extracts, we demonstrate that these proteins form a stable complex with the conserved kinetochore component hMis12. Human or chicken cells depleted of Mis12 complex subunits are delayed in mitosis with misaligned chromosomes and defects in chromosome biorientation. Aligned chromosomes exhibited reduced centromere stretch and diminished kinetochore microtubule bundles. Consistent with this, localization of the outer plate constituent Ndc80HEC1 was severely reduced. The checkpoint protein BubR1, the fibrous corona component centromere protein (CENP) E, and the inner kinetochore proteins CENP-A and CENP-H also failed to accumulate to wild-type levels in depleted cells. These results indicate that a four-subunit Mis12 complex plays an essential role in chromosome segregation in vertebrates and contributes to mitotic kinetochore assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.