ArticleBMC genomics2006
Multi-level gene expression profiles affected by thymidylate synthase and 5-fluorouracil in colon cancer.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Genomic and epigenomic biomarkers in colorectal cancer: From diagnosis to therapy.Advances in cancer research · 2021Pooled it
- SUMO1 degrader induces ER stress and ROS accumulation through deSUMOylation of TCF4 and inhibition of its transcription of StarD7 in colon cancer.Molecular carcinogenesis · 2023Article
- Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance.Nature communications · 2022Article
- Thymidylate synthase O-GlcNAcylation: a molecular mechanism of 5-FU sensitization in colorectal cancer.Oncogene · 2022Article
- ORC6 acts as a biomarker and reflects poor outcome in clear cell renal cell carcinoma.Journal of Cancer · 2022Article
- Novel candidate biomarkers of origin recognition complex 1, 5 and 6 for survival surveillance in patients with hepatocellular carcinoma.Journal of Cancer · 2020Article
- Sensitization of hepatocellular carcinoma cells towards doxorubicin and sorafenib is facilitated by glucosedependent alterations in reactive oxygen species, P-glycoprotein and DKK4.Journal of biosciences · 2020Article
- Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Translational reprogramming of colorectal cancer cells induced by 5-fluorouracil through a miRNA-dependent mechanism.Oncotarget · 2017Article
- Thymidylate synthase gene polymorphism and survival of colorectal cancer patients receiving adjuvant 5-fluorouracil.Genetic testing and molecular biomarkers · 2013Article
- The Lipid Transfer Protein StarD7: Structure, Function, and Regulation.International journal of molecular sciences · 2013Article
- Inhibition of BRCA2 and Thymidylate Synthase Creates Multidrug Sensitive Tumor Cells via the Induction of Combined "Complementary Lethality".Molecular therapy. Nucleic acids · 2013Article
- Thymidylate synthase gene (TYMS) polymorphisms in sporadic and hereditary breast cancer.BMC research notes · 2012Article
- Translational regulation of Anopheles gambiae mRNAs in the midgut during Plasmodium falciparum infection.BMC genomics · 2012Article
- TS gene polymorphisms are not good markers of response to 5-FU therapy in stage III colon cancer patients.Cellular oncology (Dordrecht, Netherlands) · 2011Article
- Cyclin E and histone H3 levels are regulated by 5-fluorouracil in a DNA mismatch repair-dependent manner.Cancer biology & therapy · 2010Article
- Integrative functional genomics analysis of sustained polyploidy phenotypes in breast cancer cells identifies an oncogenic profile for GINS2.Neoplasia (New York, N.Y.) · 2010Article
- Reduction of Orc6 expression sensitizes human colon cancer cells to 5-fluorouracil and cisplatin.PloS one · 2008Article
- Global comparative gene expression analysis of melanoma patient samples, derived cell lines and corresponding tumor xenografts.Cancer genomics & proteomicsArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThymidylate synthase (TS) is a critical target for cancer chemotherapy and is one of the most extensively studied biomarkers for fluoropyrimidine-based chemotherapy. In addition to its critical role in enzyme catalysis, TS functions as an RNA binding protein to regulate the expression of its own mRNA translation and other cellular mRNAs, such as p53, at the translational level. In this study, a comprehensive gene expression analysis at the levels of both transcriptional and post-transcriptional regulation was conducted to identify response markers using human genome array with TS-depleted human colon cancer HCT-C18 (TS-) cells and HCT-C18 (TS+) cells stably transfected with the human TS cDNA expression plasmid.
resultsA total of 38 genes were found to be significantly affected by TS based on the expression profiles of steady state mRNA transcripts. However, based on the expression profiles of polysome associated mRNA transcripts, over 149 genes were affected by TS overexpression. This indicates that additional post-transcriptionally controlled genes can be captured with profiling polysome associated mRNA population. This unique approach provides a comprehensive overview of genes affected by TS. Additional novel post-transcriptionally regulated genes affected by 5-fluorouracil (5-FU) treatment were also discovered via similar approach.
conclusionTo our knowledge, this is the first time that a comprehensive gene expression profile regulated by TS and 5-FU was analyzed at the multiple steps of gene regulation. This study will provide candidate markers that can be potentially used for predicting therapeutic outcomes for fluoropyrimidine-based cancer chemotherapy.
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