ArticleGenetics2006
Genomic analysis of the Opi- phenotype.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Pho84/GLUT2 are cellular myo-inositol exporters regulated by inositol pyrophosphates.The EMBO journal · 2026Article
- A Novel ILP Framework to Identify Compensatory Pathways in Genetic Interaction Networks with GIDEON.bioRxiv : the preprint server for biology · 2026Article
- A chimeric rather than native secretion signal elicits stress-related transcriptional adaption during 75k γ-secalin production inFEMS microbes · 2026Article
- The Inner Nuclear Membrane Has a Unique Lipid Signature.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- N88S seipin-related seipinopathy is a lipidopathy associated with loss of iron homeostasis.Cell communication and signaling : CCS · 2025Article
- Phosphatidylcholine biosynthesis pathways inFrontiers in cellular and infection microbiology · 2025Article
- Genome-Scale Screening ofJournal of fungi (Basel, Switzerland) · 2024Article
- Vitamin requirements and biosynthesis in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2020Review
- Trehalose-6-phosphate promotes fermentation and glucose repression inMicrobial cell (Graz, Austria) · 2018Article
- NuA4 Lysine Acetyltransferase Complex Contributes to Phospholipid Homeostasis inG3 (Bethesda, Md.) · 2017Article
- Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast.G3 (Bethesda, Md.) · 2017Article
- Lipid synthesis and membrane contact sites: a crossroads for cellular physiology.Journal of lipid research · 2016Review
- Budding Yeast: An Ideal Backdrop forFrontiers in cell and developmental biology · 2016Review
- The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites.The Journal of cell biology · 2015Article
- Saccharomyces cerevisiae essential genes with an Opi- phenotype.G3 (Bethesda, Md.) · 2014Article
- Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production.Molecular microbiology · 2012Article
- SCS3 and YFT2 link transcription of phospholipid biosynthetic genes to ER stress and the UPR.PLoS genetics · 2012Article
- Review
- Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.Eukaryotic cell · 2010Article
- Systematic genetic array analysis links the Saccharomyces cerevisiae SAGA/SLIK and NuA4 component Tra1 to multiple cellular processes.BMC genetics · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most of the phospholipid biosynthetic genes of Saccharomyces cerevisiae are coordinately regulated in response to inositol and choline. Inositol affects the intracellular levels of phosphatidic acid (PA). Opi1p is a repressor of the phospholipid biosynthetic genes and specifically binds PA in the endoplasmic reticulum. In the presence of inositol, PA levels decrease, releasing Opi1p into the nucleus where it represses transcription. The opi1 mutant overproduces and excretes inositol into the growth medium in the absence of inositol and choline (Opi(-) phenotype). To better understand the mechanism of Opi1p repression, the viable yeast deletion set was screened to identify Opi(-) mutants. In total, 89 Opi(-) mutants were identified, of which 7 were previously known to have the Opi(-) phenotype. The Opi(-) mutant collection included genes with roles in phospholipid biosynthesis, transcription, protein processing/synthesis, and protein trafficking. Included in this set were all nonessential components of the NuA4 HAT complex and six proteins in the Rpd3p-Sin3p HDAC complex. It has previously been shown that defects in phosphatidylcholine synthesis (cho2 and opi3) yield the Opi(-) phenotype because of a buildup of PA. However, in this case the Opi(-) phenotype is conditional because PA can be shuttled through a salvage pathway (Kennedy pathway) by adding choline to the growth medium. Seven new mutants present in the Opi(-) collection (fun26, kex1, nup84, tps1, mrpl38, mrpl49, and opi10/yol032w) were also suppressed by choline, suggesting that these affect PC synthesis. Regulation in response to inositol is also coordinated with the unfolded protein response (UPR). Consistent with this, several Opi(-) mutants were found to affect the UPR (yhi9, ede1, and vps74).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.