ArticlePlant physiology2006
Identification of a vacuolar sucrose transporter in barley and Arabidopsis mesophyll cells by a tonoplast proteomic approach.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 310 citations in OpenAlex.
- Resolving subcellular sucrose concentrations in plant tissues.Journal of experimental botany · 2026Review
- Plant SUT Sucrose Transporters: Structure, Evolution and Biological Functions.Current protein & peptide science · 2026Review
- Genome-wide identification and evolutionary analysis of SUT genes reveals key regulators of drought stress response in finger millet (Eleusine coracana).Journal, genetic engineering & biotechnology · 2025Article
- The Sugar Transporter Gene Family in Colored Calla Lily: Identification, Expression Patterns, and Roles in Soft Rot Disease.Plants (Basel, Switzerland) · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Cloning, Expression and Functional Characterization ofInternational journal of molecular sciences · 2025Article
- Genome-wide identification and expression profiling of MST, SUT and SWEET transporters in Dendrobium catenatum.BMC genomics · 2024Article
- The role of sugar transporters in the battle for carbon between plants and pathogens.Plant biotechnology journal · 2024Review
- Sugar import mediated by sugar transporters and cell wall invertases for seed development inHorticulture research · 2024Article
- Current perspectives on the regulatory mechanisms of sucrose accumulation in sugarcane.Heliyon · 2024Review
- Vacuolar proteomic analysis reveals tonoplast transporters for accumulation of citric acid and sugar in citrus fruit.Horticulture research · 2024Article
- Single-cell RNA-seq reveals a link of ovule abortion and sugar transport inFrontiers in plant science · 2024Article
- Structure and sucrose binding mechanism of the plant SUC1 sucrose transporter.Nature plants · 2023Article
- Effects of weak static magnetic fields on the development of seedlings of Arabidopsis thaliana.Protoplasma · 2023Article
- Sugar accumulation enhancement in sorghum stem is associated with reduced reproductive sink strength and increased phloem unloading activity.Frontiers in plant science · 2023Article
- How pollen tubes fight for food: the impact of sucrose carriers and invertases ofFrontiers in plant science · 2023Article
- Cold Tolerance ofCurrent issues in molecular biology · 2022Article
- Outlier analyses and genome-wide association study identifyFrontiers in plant science · 2022Article
- ISTRF: Identification of sucrose transporter using random forest.Frontiers in genetics · 2022Article
- Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries.International journal of molecular sciences · 2021Article
70 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vacuole is the main cellular storage pool, where sucrose (Suc) accumulates to high concentrations. While a limited number of vacuolar membrane proteins, such as V-type H(+)-ATPases and H(+)-pyrophosphatases, are well characterized, the majority of vacuolar transporters are still unidentified, among them the transporter(s) responsible for vacuolar Suc uptake and release. In search of novel tonoplast transporters, we used a proteomic approach, analyzing the tonoplast fraction of highly purified mesophyll vacuoles of the crop plant barley (Hordeum vulgare). We identified 101 proteins, including 88 vacuolar and putative vacuolar proteins. The Suc transporter (SUT) HvSUT2 was discovered among the 40 vacuolar proteins, which were previously not reported in Arabidopsis (Arabidopsis thaliana) vacuolar proteomic studies. To confirm the tonoplast localization of this Suc transporter, we constructed and expressed green fluorescent protein (GFP) fusion proteins with HvSUT2 and its closest Arabidopsis homolog, AtSUT4. Transient expression of HvSUT2-GFP and AtSUT4-GFP in Arabidopsis leaves and onion (Allium cepa) epidermal cells resulted in green fluorescence at the tonoplast, indicating that these Suc transporters are indeed located at the vacuolar membrane. Using a microcapillary, we selected mesophyll protoplasts from a leaf protoplast preparation and demonstrated unequivocally that, in contrast to the companion cell-specific AtSUC2, HvSUT2 and AtSUT4 are expressed in mesophyll protoplasts, suggesting that HvSUT2 and AtSUT4 are involved in transport and vacuolar storage of photosynthetically derived Suc.
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